Antibacterial activity of ovatodiolide isolated from Anisomeles indica against Helicobacter pylori

Hsiu Man Lien; Hui Yu Wu; Chiu Lien Hung; Chih Jung Chen; Chia Lin Wu; Kuan Wen Chen; Chao Lu Huang; Sheau Jiun Chang; Chia Chang Chen; Hwai Jeng Lin; Chih Ho Lai

doi:10.1038/s41598-019-40735-y

Antibacterial activity of ovatodiolide isolated from Anisomeles indica against Helicobacter pylori

Hsiu Man Lien, Hui Yu Wu, Chiu Lien Hung, Chih Jung Chen, Chia Lin Wu, Kuan Wen Chen, Chao Lu Huang, Sheau Jiun Chang, Chia Chang Chen, Hwai Jeng Lin, Chih Ho Lai

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Helicobacter pylori infection is associated with high incidence of gastric diseases. The extensive therapy of H. pylori infection with antibiotics has increased its resistance rates worldwide. Ovatodiolide, a pure constituent isolated from Anisomeles indica, has been demonstrated to possess bactericidal activity against H. pylori. In this study, ovatodiolide inhibited the growth of both H. pylori reference strain and clinical multidrug-resistant isolates. Docking analysis revealed that ovatodiolide fits into the hydrophobic pocket of a ribosomal protein, RpsB. Furthermore, ovatodiolide inhibited bacterial growth by reducing levels of RpsB, which plays a crucial role in protein translation. Our results demonstrate that ovatodiolide binds to a ribosomal protein and interferes with protein synthesis. This study provides evidence that ovatodiolide has the potential to be developed into a potent therapeutic agent for treating H. pylori infection.

Original language	English
Article number	4205
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-40735-y
Publication status	Published - Dec 1 2019

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title = "Antibacterial activity of ovatodiolide isolated from Anisomeles indica against Helicobacter pylori",

abstract = "Helicobacter pylori infection is associated with high incidence of gastric diseases. The extensive therapy of H. pylori infection with antibiotics has increased its resistance rates worldwide. Ovatodiolide, a pure constituent isolated from Anisomeles indica, has been demonstrated to possess bactericidal activity against H. pylori. In this study, ovatodiolide inhibited the growth of both H. pylori reference strain and clinical multidrug-resistant isolates. Docking analysis revealed that ovatodiolide fits into the hydrophobic pocket of a ribosomal protein, RpsB. Furthermore, ovatodiolide inhibited bacterial growth by reducing levels of RpsB, which plays a crucial role in protein translation. Our results demonstrate that ovatodiolide binds to a ribosomal protein and interferes with protein synthesis. This study provides evidence that ovatodiolide has the potential to be developed into a potent therapeutic agent for treating H. pylori infection.",

author = "Lien, {Hsiu Man} and Wu, {Hui Yu} and Hung, {Chiu Lien} and Chen, {Chih Jung} and Wu, {Chia Lin} and Chen, {Kuan Wen} and Huang, {Chao Lu} and Chang, {Sheau Jiun} and Chen, {Chia Chang} and Lin, {Hwai Jeng} and Lai, {Chih Ho}",

note = "Funding Information: The authors would like to thank the editor and reviewers for the editorial assistance and their valuable comments. The authors thank Yushen Biotech Co. (Taichung, Taiwan) for providing ovatodiolide. This study is supported in part by Taiwan Ministry of Science and Technology (105-2313-B-182-001 and 106-2320-B-182-012-MY3), Chang Gung Memorial Hospital (CMRPD1F0011-3, CMRPD1F0431-3, CMRPD1I0061, and BMRPE90), and Tomorrow Medical Foundation. Publisher Copyright: {\textcopyright} 2019, The Author(s).",

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doi = "10.1038/s41598-019-40735-y",

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T1 - Antibacterial activity of ovatodiolide isolated from Anisomeles indica against Helicobacter pylori

AU - Lien, Hsiu Man

AU - Wu, Hui Yu

AU - Hung, Chiu Lien

AU - Chen, Chih Jung

AU - Wu, Chia Lin

AU - Chen, Kuan Wen

AU - Huang, Chao Lu

AU - Chang, Sheau Jiun

AU - Chen, Chia Chang

AU - Lin, Hwai Jeng

AU - Lai, Chih Ho

N1 - Funding Information: The authors would like to thank the editor and reviewers for the editorial assistance and their valuable comments. The authors thank Yushen Biotech Co. (Taichung, Taiwan) for providing ovatodiolide. This study is supported in part by Taiwan Ministry of Science and Technology (105-2313-B-182-001 and 106-2320-B-182-012-MY3), Chang Gung Memorial Hospital (CMRPD1F0011-3, CMRPD1F0431-3, CMRPD1I0061, and BMRPE90), and Tomorrow Medical Foundation. Publisher Copyright: © 2019, The Author(s).

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Helicobacter pylori infection is associated with high incidence of gastric diseases. The extensive therapy of H. pylori infection with antibiotics has increased its resistance rates worldwide. Ovatodiolide, a pure constituent isolated from Anisomeles indica, has been demonstrated to possess bactericidal activity against H. pylori. In this study, ovatodiolide inhibited the growth of both H. pylori reference strain and clinical multidrug-resistant isolates. Docking analysis revealed that ovatodiolide fits into the hydrophobic pocket of a ribosomal protein, RpsB. Furthermore, ovatodiolide inhibited bacterial growth by reducing levels of RpsB, which plays a crucial role in protein translation. Our results demonstrate that ovatodiolide binds to a ribosomal protein and interferes with protein synthesis. This study provides evidence that ovatodiolide has the potential to be developed into a potent therapeutic agent for treating H. pylori infection.

AB - Helicobacter pylori infection is associated with high incidence of gastric diseases. The extensive therapy of H. pylori infection with antibiotics has increased its resistance rates worldwide. Ovatodiolide, a pure constituent isolated from Anisomeles indica, has been demonstrated to possess bactericidal activity against H. pylori. In this study, ovatodiolide inhibited the growth of both H. pylori reference strain and clinical multidrug-resistant isolates. Docking analysis revealed that ovatodiolide fits into the hydrophobic pocket of a ribosomal protein, RpsB. Furthermore, ovatodiolide inhibited bacterial growth by reducing levels of RpsB, which plays a crucial role in protein translation. Our results demonstrate that ovatodiolide binds to a ribosomal protein and interferes with protein synthesis. This study provides evidence that ovatodiolide has the potential to be developed into a potent therapeutic agent for treating H. pylori infection.

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Antibacterial activity of ovatodiolide isolated from Anisomeles indica against Helicobacter pylori

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