Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19

Yu Li Chen; Chun Yu Chen; Kuei Hung Lai; Yu Chia Chang; Tsong Long Hwang

doi:10.1016/j.jff.2023.105407

Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19

Yu Li Chen
, Chun Yu Chen
, Kuei Hung Lai
, Yu Chia Chang
, Tsong Long Hwang

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Lophatherum gracile (L. gracile) has long been used as a functional food and herbal medicine. Previous studies have demonstrated that extracts of L. gracile attenuate inflammatory response and inhibit SARS-CoV-2 replication; however, the underlying active constituents have yet to be identified. This study investigated the bioactive components of L. gracile. Flavone C-glycosides of L. gracile were found to dominate both anti-inflammatory and antiviral effects. A simple chromatography-based method was developed to obtain flavone C-glycoside-enriched extract (FlavoLG) from L. gracile. FlavoLG and its major flavone C-glycoside isoorientin were shown to restrict respiratory bursts and the formation of neutrophil extracellular traps in activated human neutrophils. FlavoLG and isoorientin were also shown to inhibit SARS-CoV-2 pseudovirus infection by interfering with the binding of the SARS-CoV-2 spike on ACE2. These results provide scientific evidence indicating the efficacy of L. gracile as a potential supplement for treating neutrophil-associated COVID-19.

Original language	English
Article number	105407
Journal	Journal of Functional Foods
Volume	101
DOIs	https://doi.org/10.1016/j.jff.2023.105407
Publication status	Published - Feb 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

COVID-19
Flavone C-glycosides
Lophatherum gracile
Neutrophils
SARS-CoV-2

ASJC Scopus subject areas

Medicine (miscellaneous)
Food Science
Nutrition and Dietetics

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10.1016/j.jff.2023.105407

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@article{d96b7b14bbeb4d348eea577cb0d44348,

title = "Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19",

abstract = "Lophatherum gracile (L. gracile) has long been used as a functional food and herbal medicine. Previous studies have demonstrated that extracts of L. gracile attenuate inflammatory response and inhibit SARS-CoV-2 replication; however, the underlying active constituents have yet to be identified. This study investigated the bioactive components of L. gracile. Flavone C-glycosides of L. gracile were found to dominate both anti-inflammatory and antiviral effects. A simple chromatography-based method was developed to obtain flavone C-glycoside-enriched extract (FlavoLG) from L. gracile. FlavoLG and its major flavone C-glycoside isoorientin were shown to restrict respiratory bursts and the formation of neutrophil extracellular traps in activated human neutrophils. FlavoLG and isoorientin were also shown to inhibit SARS-CoV-2 pseudovirus infection by interfering with the binding of the SARS-CoV-2 spike on ACE2. These results provide scientific evidence indicating the efficacy of L. gracile as a potential supplement for treating neutrophil-associated COVID-19.",

keywords = "COVID-19, Flavone C-glycosides, Lophatherum gracile, Neutrophils, SARS-CoV-2",

author = "Chen, \{Yu Li\} and Chen, \{Chun Yu\} and Lai, \{Kuei Hung\} and Chang, \{Yu Chia\} and Hwang, \{Tsong Long\}",

note = "Funding Information: This work was supported by grants from the Chang Gung Memorial Hospital (CMRPG3K0921, CMRPF1L0071, CMRPF1M0101, CMRPF1M0131, CMRPF1M0091 and CORPF1L0011), the Chang Gung University of Science and Technology (ZRRPF3L0091), and the National Science and Technology Council (108{\textendash}2320-B-255{\textendash}003-MY3, 109{\textendash}2811-B-255{\textendash}502, 109{\textendash}2327-B-255{\textendash}001, 111{\textendash}2321-B-255{\textendash}001, 111{\textendash}2320-B-255{\textendash}006-MY3, 111{\textendash}2811-B-255{\textendash}002, 111{\textendash}2321-B-182{\textendash}001). Funding Information: We would like to thank the National RNAi Core Facility at Academia Sinica in Taiwan for providing the SARS-CoV-2 pseudovirus and related services. We thank Prof. Rei-Lin Kuo (Chang Gung University) for kindly providing hACE-2-overexpressed HEK293T cells. Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = feb,

doi = "10.1016/j.jff.2023.105407",

language = "English",

volume = "101",

journal = "Journal of Functional Foods",

issn = "1756-4646",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19

AU - Chen, Yu Li

AU - Chen, Chun Yu

AU - Lai, Kuei Hung

AU - Chang, Yu Chia

AU - Hwang, Tsong Long

N1 - Funding Information: This work was supported by grants from the Chang Gung Memorial Hospital (CMRPG3K0921, CMRPF1L0071, CMRPF1M0101, CMRPF1M0131, CMRPF1M0091 and CORPF1L0011), the Chang Gung University of Science and Technology (ZRRPF3L0091), and the National Science and Technology Council (108–2320-B-255–003-MY3, 109–2811-B-255–502, 109–2327-B-255–001, 111–2321-B-255–001, 111–2320-B-255–006-MY3, 111–2811-B-255–002, 111–2321-B-182–001). Funding Information: We would like to thank the National RNAi Core Facility at Academia Sinica in Taiwan for providing the SARS-CoV-2 pseudovirus and related services. We thank Prof. Rei-Lin Kuo (Chang Gung University) for kindly providing hACE-2-overexpressed HEK293T cells. Publisher Copyright: © 2023 The Authors

PY - 2023/2

Y1 - 2023/2

N2 - Lophatherum gracile (L. gracile) has long been used as a functional food and herbal medicine. Previous studies have demonstrated that extracts of L. gracile attenuate inflammatory response and inhibit SARS-CoV-2 replication; however, the underlying active constituents have yet to be identified. This study investigated the bioactive components of L. gracile. Flavone C-glycosides of L. gracile were found to dominate both anti-inflammatory and antiviral effects. A simple chromatography-based method was developed to obtain flavone C-glycoside-enriched extract (FlavoLG) from L. gracile. FlavoLG and its major flavone C-glycoside isoorientin were shown to restrict respiratory bursts and the formation of neutrophil extracellular traps in activated human neutrophils. FlavoLG and isoorientin were also shown to inhibit SARS-CoV-2 pseudovirus infection by interfering with the binding of the SARS-CoV-2 spike on ACE2. These results provide scientific evidence indicating the efficacy of L. gracile as a potential supplement for treating neutrophil-associated COVID-19.

AB - Lophatherum gracile (L. gracile) has long been used as a functional food and herbal medicine. Previous studies have demonstrated that extracts of L. gracile attenuate inflammatory response and inhibit SARS-CoV-2 replication; however, the underlying active constituents have yet to be identified. This study investigated the bioactive components of L. gracile. Flavone C-glycosides of L. gracile were found to dominate both anti-inflammatory and antiviral effects. A simple chromatography-based method was developed to obtain flavone C-glycoside-enriched extract (FlavoLG) from L. gracile. FlavoLG and its major flavone C-glycoside isoorientin were shown to restrict respiratory bursts and the formation of neutrophil extracellular traps in activated human neutrophils. FlavoLG and isoorientin were also shown to inhibit SARS-CoV-2 pseudovirus infection by interfering with the binding of the SARS-CoV-2 spike on ACE2. These results provide scientific evidence indicating the efficacy of L. gracile as a potential supplement for treating neutrophil-associated COVID-19.

KW - COVID-19

KW - Flavone C-glycosides

KW - Lophatherum gracile

KW - Neutrophils

KW - SARS-CoV-2

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UR - https://www.scopus.com/pages/publications/85146041234#tab=citedBy

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DO - 10.1016/j.jff.2023.105407

M3 - Article

AN - SCOPUS:85146041234

SN - 1756-4646

VL - 101

JO - Journal of Functional Foods

JF - Journal of Functional Foods

M1 - 105407

ER -

Anti-inflammatory and antiviral activities of flavone C-glycosides of Lophatherum gracile for COVID-19

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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