TY - JOUR
T1 - Anti-fouling and anti-coagulation capabilities of PEDOT-biopolymer coating by in-situ electrochemical copolymerization
AU - Hsu, Chuan Chih
AU - Liu, Ting Yu
AU - Peng, Xin Yao
AU - Cheng, Yu Wei
AU - Lin, Yan Ru
AU - Yang, Ming Chien
AU - Huang, Li Ying
AU - Liu, Kun Ho
AU - Yung, Ming Chi
N1 - Funding Information:
This study was financially supported by the Taiwan Association of Cardiovascular Surgery Research and Ministry of Science and Technology of Taiwan (MOST 104-2221-E-011-089-MY3, MOST 107-2221-E-011-042, and MOST 108-2622-E-131-002-CC3). Platelet-poor plasma and platelet-rich plasma were provided by the Taipei Blood Center of Taiwan. The IRB number (201702016RINB) was provided by Dr. Juey-Jen Hwang. The assistance provided by the National Taiwan University Hospital is also gratefully acknowledged.
Funding Information:
This study was financially supported by the Taiwan Association of Cardiovascular Surgery Research and Ministry of Science and Technology of Taiwan ( MOST 104-2221-E-011-089-MY3 , MOST 107-2221-E-011-042 , and MOST 108-2622-E-131-002-CC3 ). Platelet-poor plasma and platelet-rich plasma were provided by the Taipei Blood Center of Taiwan. The IRB number (201702016RINB) was provided by Dr. Juey-Jen Hwang. The assistance provided by the National Taiwan University Hospital is also gratefully acknowledged.
Publisher Copyright:
© 2020
PY - 2020/9/15
Y1 - 2020/9/15
N2 - In this study, 3,4-ethylenedioxythiophene (EDOT), polystyrene sulfonate (PSS), chondroitin sulfate (CHS), and carboxymethyl-hexanoyl chitosan (CHC) nanohybrids were coated on nitrogen plasma pre-treated SUS316L stainless steel using in-situ electrochemically copolymerization. The negatively charged PSS, CHS, and CHC were used to repel the negatively charged proteins, platelets, and cell walls of bacteria (E. coli) to achieve anti-fouling and anti-clotting capabilities. The anti-fouling capabilities of the poly-3,4-ethylenedioxythiophene (PEDOT) derivative coatings had the following order: PEDOT/CHC/CHS > PEDOT/CHS > PEDOT/CHS/PSS > PEDOT/PSS > pristine SUS316L. In particular, with the addition of CHC, the PEDOT/CHC/CHS coating could decrease the adhesion of platelets and bacteria using the functional group (-COOH) of CHC. The blood clotting time (activated partial thromboplastin time) of the PEDOT/CHC/CHS substrates was prolonged to 48 s, compared to the 32 s required for the pristine SUS316L stainless steel, showing the good hemocompatibility. Furthermore, cytotoxicity tests showed that PEDOT and its derivatives exhibited very good biocompatibility. Thus, they could potentially be applied in anti-clotting, anti-fouling, and anti-bacterial biomedical devices such as cardiovascular stents.
AB - In this study, 3,4-ethylenedioxythiophene (EDOT), polystyrene sulfonate (PSS), chondroitin sulfate (CHS), and carboxymethyl-hexanoyl chitosan (CHC) nanohybrids were coated on nitrogen plasma pre-treated SUS316L stainless steel using in-situ electrochemically copolymerization. The negatively charged PSS, CHS, and CHC were used to repel the negatively charged proteins, platelets, and cell walls of bacteria (E. coli) to achieve anti-fouling and anti-clotting capabilities. The anti-fouling capabilities of the poly-3,4-ethylenedioxythiophene (PEDOT) derivative coatings had the following order: PEDOT/CHC/CHS > PEDOT/CHS > PEDOT/CHS/PSS > PEDOT/PSS > pristine SUS316L. In particular, with the addition of CHC, the PEDOT/CHC/CHS coating could decrease the adhesion of platelets and bacteria using the functional group (-COOH) of CHC. The blood clotting time (activated partial thromboplastin time) of the PEDOT/CHC/CHS substrates was prolonged to 48 s, compared to the 32 s required for the pristine SUS316L stainless steel, showing the good hemocompatibility. Furthermore, cytotoxicity tests showed that PEDOT and its derivatives exhibited very good biocompatibility. Thus, they could potentially be applied in anti-clotting, anti-fouling, and anti-bacterial biomedical devices such as cardiovascular stents.
KW - Anti-coagulation
KW - Anti-fouling
KW - Carboxymethyl-hexanoyl chitosan
KW - Chondroitin sulfate
KW - Electrochemical polymerization
KW - PEDOT
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UR - http://www.scopus.com/inward/citedby.url?scp=85085684059&partnerID=8YFLogxK
U2 - 10.1016/j.surfcoat.2020.125963
DO - 10.1016/j.surfcoat.2020.125963
M3 - Article
AN - SCOPUS:85085684059
SN - 0257-8972
VL - 397
JO - Surface and Coatings Technology
JF - Surface and Coatings Technology
M1 - 125963
ER -