Anti-dengue virus nonstructural protein 1 antibodies contribute to platelet phagocytosis by macrophages

Shu Wen Wan, Yi Wen Yang, Ya Ting Chu, Chiou Feng Lin, Chih Peng Chang, Trai Ming Yeh, Robert Anderson, Yee Shin Lin

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Thrombocytopenia is an important clinical manifestation of dengue disease. The hypotheses concerning the pathogenesis of thrombocytopenia include decreased production and increased destruction or consumption of platelets. We previously suggested a mechanism of molecular mimicry in which antibodies (Abs) directed against dengue virus (DENV) nonstructural protein 1 (NS1) cross-react with platelets. Furthermore, several lines of evidence show activation of endothelial cells (ECs) and macrophages are related to dengue disease severity. Previous studies also suggested that Ab-opsonised platelets facilitate the engulfment of platelets by macrophages. Here we show that TNFα-activated ECs upregulate adhesion molecule expression to enhance the binding of platelets and macrophages and lead to anti-DENV NS1 Ab-mediated platelet phagocytosis. We further demonstrate that the interaction between macrophages and TNF-α-activated ECs requires binding of FcγR with the Fc region of platelet-bound anti-DENV NS1 Abs. Importantly, the binding of anti-DENV NS1 Abs to platelets did not interfere with platelet adhesion to ECs. The adhesion molecules ICAM-1 and β3 integrin expressed on ECs as well as the FcγR expressed on macrophages were critical in anti-DENV NS1 Ab-mediated platelet phagocytosis on activated ECs. Moreover, anti-DENV NS1 Abs dramatically enhanced platelet engulfment by macrophages in a murine model of DENV infection. Our study provides evidence for a novel role for anti-DENV NS1 Abs in the pathogenesis of thrombocytopenia in dengue disease by enhancing platelet phagocytosis by macrophages.

Original languageEnglish
Pages (from-to)646-656
Number of pages11
JournalThrombosis and Haemostasis
Issue number3
Publication statusPublished - 2016


  • Dengue virus
  • Nonstructural protein 1
  • Platelet phagocytosis

ASJC Scopus subject areas

  • Hematology


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