Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

Chien-Neng Kuo; Ching-Hsein Chen; San-Ni Chen; Jou-Cheng Huang; Li-Ju Lai; Chien-Hsiung Lai; Chia-Hui Hung; Chien-Hsing Lee; Chung-Yi Chen

doi:10.1007/s10792-017-0530-6

Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

Chien-Neng Kuo
, Ching-Hsein Chen
, San-Ni Chen
, Jou-Cheng Huang
, Li-Ju Lai
, Chien-Hsiung Lai
, Chia-Hui Hung
, Chien-Hsing Lee
, Chung-Yi Chen

Department of Ophthalmology

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

AIM: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.

METHODS: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.

RESULTS: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.

CONCLUSIONS: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.

Original language	English
Pages (from-to)	747-756
Number of pages	10
Journal	International Ophthalmology
Volume	38
Issue number	2
DOIs	https://doi.org/10.1007/s10792-017-0530-6
Publication status	Published - Apr 2018

Keywords

Angiogenesis Inhibitors/pharmacology
Animals
Blotting, Western
Conjunctiva/drug effects
Corneal Neovascularization/drug therapy
Curcumin/analogs & derivatives
Disease Models, Animal
Fibroblast Growth Factor 2/metabolism
HLA-DR Antigens/metabolism
Male
Rats
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A/metabolism

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10.1007/s10792-017-0530-6

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title = "Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization",

abstract = "AIM: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 \& p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.METHODS: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 \& p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.RESULTS: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.CONCLUSIONS: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.",

keywords = "Angiogenesis Inhibitors/pharmacology, Animals, Blotting, Western, Conjunctiva/drug effects, Corneal Neovascularization/drug therapy, Curcumin/analogs \& derivatives, Disease Models, Animal, Fibroblast Growth Factor 2/metabolism, HLA-DR Antigens/metabolism, Male, Rats, Rats, Sprague-Dawley, Vascular Endothelial Growth Factor A/metabolism",

author = "Chien-Neng Kuo and Ching-Hsein Chen and San-Ni Chen and Jou-Cheng Huang and Li-Ju Lai and Chien-Hsiung Lai and Chia-Hui Hung and Chien-Hsing Lee and Chung-Yi Chen",

year = "2018",

month = apr,

doi = "10.1007/s10792-017-0530-6",

language = "English",

volume = "38",

pages = "747--756",

journal = "International Ophthalmology",

issn = "0165-5701",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

AU - Kuo, Chien-Neng

AU - Chen, Ching-Hsein

AU - Chen, San-Ni

AU - Huang, Jou-Cheng

AU - Lai, Li-Ju

AU - Lai, Chien-Hsiung

AU - Hung, Chia-Hui

AU - Lee, Chien-Hsing

AU - Chen, Chung-Yi

PY - 2018/4

Y1 - 2018/4

N2 - AIM: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.METHODS: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.RESULTS: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.CONCLUSIONS: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.

AB - AIM: This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.METHODS: CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.RESULTS: Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.CONCLUSIONS: Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.

KW - Angiogenesis Inhibitors/pharmacology

KW - Animals

KW - Blotting, Western

KW - Conjunctiva/drug effects

KW - Corneal Neovascularization/drug therapy

KW - Curcumin/analogs & derivatives

KW - Disease Models, Animal

KW - Fibroblast Growth Factor 2/metabolism

KW - HLA-DR Antigens/metabolism

KW - Male

KW - Rats

KW - Rats, Sprague-Dawley

KW - Vascular Endothelial Growth Factor A/metabolism

U2 - 10.1007/s10792-017-0530-6

DO - 10.1007/s10792-017-0530-6

M3 - Article

C2 - 28393322

SN - 0165-5701

VL - 38

SP - 747

EP - 756

JO - International Ophthalmology

JF - International Ophthalmology

IS - 2

ER -

Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

Abstract

Keywords

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