Angiotensinogen gene expression is induced by cyclical mechanical stretch in cultured rat cardiomyocytes

K. G. Shyu, J. J. Chen, N. L. Shih, H. Chang, D. L. Wang, W. P. Lien, C. C. Liew

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48 Citations (Scopus)


The effect of cyclical mechanical stretch on angiotensinogen gene expression was examined using a neonatal rat cardiocyte culture system. Cultured cardiocytes grown on a flexible membrane base were stretched by vacuum to 20% of maximum elongation, at 60 cycles/min. The angiotensinogen gene was activated 2 to 5 fold after stretch for 3 to 24 hr, as shown by quantitative reverse transcription polymerase chain reaction. The 5'-flanking region of the angiotensinogen promoter was activated after stretch for 24 hr. This gene expression could be completely suppressed by losartan, a specific antagonist of angiotensin II receptor. These results indicate that (1) cyclical mechanical stretching of cardiocytes is a good model for the study of cardiac hypertrophy-related gene expression; (2) cyclical stretch up-regulates expression of the angiotensinogen gene and (3) the increase in promoter activity may contribute to the induction of angiotensinogen mRNA by cyclical stretch.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology


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