Angiotensin II prompts heart cell apoptosis via AT1 receptor-augmented phosphatase and tensin homolog and miR-320-3p functions to enhance suppression of the IGF1R-PI3K-AKT survival pathway

Shang Yeh Lu, Wei Zhi Hong, Bruce Chi Kang Tsai, Yu Chun Chang, Chia Hua Kuo, Thomas G. Mhone, Ray Jade Chen, Wei Wen Kuo, Chih Yang Huang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background:Hypertension is a severe public health risk factor worldwide. Elevated angiotensin II (Ang II) produced by the renin-angiotensin-aldosterone system can lead to hypertension and its complications.Method:In this study, we addressed the cardiac-injury effects of Ang II and investigated the signaling mechanism induced by Ang II. Both H9c2 cardiomyoblast cells and neonatal rat cardiomyocytes were exposed to Ang II to observe hypertension-related cardiac apoptosis.Results:The results of western blotting revealed that Ang II significantly attenuated the IGF1R-PI3K-AKT pathway via the Ang II-AT1 receptor axis and phosphatase and tensin homolog expression. Furthermore, real-time PCR showed that Ang II also activated miR-320-3p transcription to repress the PI3K-Akt pathway. In the heart tissue of spontaneously hypertensive rats, activation of the IGF1R survival pathway was also reduced compared with that in Wistar-Kyoto rats, especially in aged spontaneously hypertensive rats.Conclusion:Hence, we speculate that the Ang II-AT1 receptor axis induces both phosphatase and tensin homolog and miR-320-3p expression to downregulate the IGF1R-PI3K-AKT survival pathway and cause cell apoptosis in the heart.

Original languageEnglish
Pages (from-to)2502-2512
Number of pages11
JournalJournal of Hypertension
Volume40
Issue number12
DOIs
Publication statusPublished - Dec 2022

Keywords

  • angiotensin II
  • heart damage
  • hypertension
  • mir-320
  • phosphatase and tensin homolog
  • renin-angiotensin-aldosterone system

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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