An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan; Ching Jiun Lee; Jen Hao Hsu; Yi Hsing Liu; Szu I. Yeh; Horn Jiunn Sheen; Shiming Lin; Jing Fa Tsai

doi:10.1109/NEMS.2010.5592441

An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan, Ching Jiun Lee, Jen Hao Hsu, Yi Hsing Liu, Szu I. Yeh, Horn Jiunn Sheen, Shiming Lin, Jing Fa Tsai

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

Original language	English
Title of host publication	2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages	487-491
Number of pages	5
DOIs	https://doi.org/10.1109/NEMS.2010.5592441
Publication status	Published - 2010
Externally published	Yes
Event	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China Duration: Jan 20 2010 → Jan 23 2010

Conference

Conference	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Country/Territory	China
City	Xiamen
Period	1/20/10 → 1/23/10

Keywords

Biosensing
Brownian motion
Dielectrophoresis (DEP)
Micro-particle tracking velocimetry (μ-PTV)

ASJC Scopus subject areas

Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/NEMS.2010.5592441

Cite this

Fan, YJ, Lee, CJ, Hsu, JH, Liu, YH, Yeh, SI, Sheen, HJ, Lin, S & Tsai, JF 2010, An integrated system of RBCs sorting and Brownian motion bio-sensing technique. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592441, pp. 487-491, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, Xiamen, China, 1/20/10. https://doi.org/10.1109/NEMS.2010.5592441

@inproceedings{c23f419154854ca59a5aaa09c5404629,

title = "An integrated system of RBCs sorting and Brownian motion bio-sensing technique",

abstract = "A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).",

keywords = "Biosensing, Brownian motion, Dielectrophoresis (DEP), Micro-particle tracking velocimetry (μ-PTV)",

author = "Fan, {Yu Jui} and Lee, {Ching Jiun} and Hsu, {Jen Hao} and Liu, {Yi Hsing} and Yeh, {Szu I.} and Sheen, {Horn Jiunn} and Shiming Lin and Tsai, {Jing Fa}",

year = "2010",

doi = "10.1109/NEMS.2010.5592441",

language = "English",

isbn = "9781424465439",

pages = "487--491",

booktitle = "2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010",

note = "5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 ; Conference date: 20-01-2010 Through 23-01-2010",

}

TY - GEN

T1 - An integrated system of RBCs sorting and Brownian motion bio-sensing technique

AU - Fan, Yu Jui

AU - Lee, Ching Jiun

AU - Hsu, Jen Hao

AU - Liu, Yi Hsing

AU - Yeh, Szu I.

AU - Sheen, Horn Jiunn

AU - Lin, Shiming

AU - Tsai, Jing Fa

PY - 2010

Y1 - 2010

N2 - A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

AB - A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

KW - Biosensing

KW - Brownian motion

KW - Dielectrophoresis (DEP)

KW - Micro-particle tracking velocimetry (μ-PTV)

UR - http://www.scopus.com/inward/record.url?scp=78649297183&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649297183&partnerID=8YFLogxK

U2 - 10.1109/NEMS.2010.5592441

DO - 10.1109/NEMS.2010.5592441

M3 - Conference contribution

AN - SCOPUS:78649297183

SN - 9781424465439

SP - 487

EP - 491

BT - 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

T2 - 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

Y2 - 20 January 2010 through 23 January 2010

ER -

An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Abstract

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this