An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan; Ching Jiun Lee; Jen Hao Hsu; Yi Hsing Liu; Szu I. Yeh; Horn Jiunn Sheen; Shiming Lin; Jing Fa Tsai

doi:10.1109/NEMS.2010.5592441

An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan, Ching Jiun Lee, Jen Hao Hsu, Yi Hsing Liu, Szu I. Yeh, Horn Jiunn Sheen, Shiming Lin, Jing Fa Tsai

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

Original language	English
Title of host publication	2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages	487-491
Number of pages	5
DOIs	https://doi.org/10.1109/NEMS.2010.5592441
Publication status	Published - 2010
Externally published	Yes
Event	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China Duration: Jan 20 2010 → Jan 23 2010

Publication series

Name	2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

Conference

Conference	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Country/Territory	China
City	Xiamen
Period	1/20/10 → 1/23/10

Keywords

Biosensing
Brownian motion
Dielectrophoresis (DEP)
Micro-particle tracking velocimetry (μ-PTV)

ASJC Scopus subject areas

Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/NEMS.2010.5592441

Cite this

Fan, Y. J., Lee, C. J., Hsu, J. H., Liu, Y. H., Yeh, S. I., Sheen, H. J., Lin, S., & Tsai, J. F. (2010). An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 487-491). Article 5592441 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592441

An integrated system of RBCs sorting and Brownian motion bio-sensing technique. / Fan, Yu Jui; Lee, Ching Jiun; Hsu, Jen Hao et al.
2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 487-491 5592441 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fan, YJ, Lee, CJ, Hsu, JH, Liu, YH, Yeh, SI, Sheen, HJ, Lin, S & Tsai, JF 2010, An integrated system of RBCs sorting and Brownian motion bio-sensing technique. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592441, 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, pp. 487-491, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, Xiamen, China, 1/20/10. https://doi.org/10.1109/NEMS.2010.5592441

Fan YJ, Lee CJ, Hsu JH, Liu YH, Yeh SI, Sheen HJ et al. An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 487-491. 5592441. (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). doi: 10.1109/NEMS.2010.5592441

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abstract = "A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).",

keywords = "Biosensing, Brownian motion, Dielectrophoresis (DEP), Micro-particle tracking velocimetry (μ-PTV)",

author = "Fan, {Yu Jui} and Lee, {Ching Jiun} and Hsu, {Jen Hao} and Liu, {Yi Hsing} and Yeh, {Szu I.} and Sheen, {Horn Jiunn} and Shiming Lin and Tsai, {Jing Fa}",

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AU - Fan, Yu Jui

AU - Lee, Ching Jiun

AU - Hsu, Jen Hao

AU - Liu, Yi Hsing

AU - Yeh, Szu I.

AU - Sheen, Horn Jiunn

AU - Lin, Shiming

AU - Tsai, Jing Fa

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AB - A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

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KW - Brownian motion

KW - Dielectrophoresis (DEP)

KW - Micro-particle tracking velocimetry (μ-PTV)

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An integrated system of RBCs sorting and Brownian motion bio-sensing technique

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Keywords

ASJC Scopus subject areas

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