An insight into nano silver fluoride-coated silk fibroin bioinspiredmembrane properties for guided tissue regeneration

Aditi Pandey, Tzu Sen Yang, Ta I. Yang, Wendimi Fatimata Belem, Nai Chia Teng, I. Wen Chen, Ching Shuan Huang, Aivaras Kareiva, Jen Chang Yang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The current work focuses on the development of a novel electrospun silk fibroin (SF) nonwoven mat as a GTR membrane with antibacterial, biomineralization and biocompatible properties. The γ-poly glutamic acid (γ-PGA)-capped nano silver fluoride (NSF) and silver diamine fluoride (SDF) were first synthesized, which were dip-coated onto electrospun silk fibroin mats (NSF-SF and SDF-SF). UV-Vis spectroscopy and TEM depicted the formation of silver nanoparticles. NSF-SF and SDF-SF demonstrated antibacterial properties (against Porphyromonas gingivalis) with 3.1 and 6.7 folds higher relative to SF, respectively. Post-mineralization in simulated body fluid, the NSF-SF effectively promoted apatite precipitation (Ca/P ~1.67), while the SDF-SF depicted deposition of silver nanoparticles, assessed by SEM-EDS. According to the FTIR-ATR deconvolution analysis, NSF-SF portrayed ~75% estimated hydroxyapatite crystallinity index (CI), whereas pure SF and SDF-SF demonstrated ~60%. The biocompatibility of NSF-SF was ~82% when compared to the control, while SDF-coated samples revealed in vitro cytotoxicity, further needing in vivo studies for a definite conclusion. Furthermore, the NSF-SF revealed the highest tensile strength of 0.32 N/mm and 1.76% elongation at break. Therefore, it is substantiated that the novel bioactive and antibacterial NSF-SF membranes can serve as a potential candidate, shedding light on further in-depth analysis for GTR applications.

Original languageEnglish
Article number2659
Issue number16
Publication statusPublished - Aug 2021


  • Antibacterial efficacy
  • Biomineralization
  • In vitro cytotoxicity
  • Nano silver fluoride
  • Silver diamine fluoride

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics


Dive into the research topics of 'An insight into nano silver fluoride-coated silk fibroin bioinspiredmembrane properties for guided tissue regeneration'. Together they form a unique fingerprint.

Cite this