Alpha coefficient of dose-response for chromosome translocations measured by FISH in human lymphocytes exposed to chronic 60Co gamma rays at body temperature

W. A. Hsieh, W. Deng, W. P. Chang, N. Galvan, C. L. Owens, D. P. Morrison, K. L. Gale, J. N. Lucas

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22 Citations (Scopus)

Abstract

Purpose: To measure the alpha coefficient, the initial slope of the translocation dose-response curve, for 60Co γ-rays in human lymphocytes. Materials and methods: Human lymphocytes were exposed to 0, 0.32, 0.62 and 0.92 Gy of chronic 60Co γ-rays under conditions that reduce the metabolic stress to the cells. Chromosome translocation frequencies were measured using fluorescence in situ hybridization with a whole-chromosome probe cocktail specific for chromosomes 1, 2, 4 (orange) and 3, 5, 6 (green). Results: A total of 72 383 metaphases were analysed (33 429 in exposed cells) in two individuals. The shape of the dose-response curves for translocations was linear, and alpha coefficient was measured as 0.024 ± 0.002 translocations per cell per Gy for the combined data for two 24 year old male donors. Conclusion: The alpha coefficients measured after chronic exposure were in good agreement with that reported in the literature for acute, low-dose exposure of human lymphocytes to 60Co γ-rays.

Original languageEnglish
Pages (from-to)435-439
Number of pages5
JournalInternational Journal of Radiation Biology
Volume75
Issue number4
DOIs
Publication statusPublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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