TY - JOUR
T1 - Allylamine coating on zirconia dental implant surface promotes osteogenic differentiation in vitro and accelerates osseointegration in vivo
AU - Salamanca, Eisner
AU - Wu, Yi Fan
AU - Aung, Lwin Moe
AU - Chiu, Bor Rong
AU - Chen, Mei Kuang
AU - Chang, Wei Jen
AU - Sun, Ying Sui
N1 - Publisher Copyright:
© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2024
Y1 - 2024
N2 - Objectives: The glow discharge plasma (GDP) procedure has proven efficacy in grafting allylamine onto zirconia dental implant surfaces to enhance osseointegration. This study explored the enhancement of zirconia dental implant properties using GDP at different energy settings (25, 50, 75, 100, and 200 W) both in vitro and in vivo. Materials and Methods: In vitro analyses included scanning electron microscopy, wettability assessment, energy-dispersive X-ray spectroscopy, and more. In vivo experiments involved implanting zirconia dental implants into rabbit femurs and later evaluation through impact stability test, micro-CT, and histomorphometric measurements. Results: The results demonstrated that 25 and 50 W GDP allylamine grafting positively impacted MG-63 cell proliferation and increased alkaline phosphatase activity. Gene expression analysis revealed upregulation of OCN, OPG, and COL-I. Both 25 and 50 W GDP allylamine grafting significantly improved zirconia's surface properties (p <.05, p <.01, p <.001). However, only 25 W allylamine grafting with optimal energy settings promoted in vivo osseointegration and new bone formation while preventing bone level loss around the dental implant (p <.05, p <.01, p <.001). Conclusions: This study presents a promising method for enhancing Zr dental implant surface's bioactivity.
AB - Objectives: The glow discharge plasma (GDP) procedure has proven efficacy in grafting allylamine onto zirconia dental implant surfaces to enhance osseointegration. This study explored the enhancement of zirconia dental implant properties using GDP at different energy settings (25, 50, 75, 100, and 200 W) both in vitro and in vivo. Materials and Methods: In vitro analyses included scanning electron microscopy, wettability assessment, energy-dispersive X-ray spectroscopy, and more. In vivo experiments involved implanting zirconia dental implants into rabbit femurs and later evaluation through impact stability test, micro-CT, and histomorphometric measurements. Results: The results demonstrated that 25 and 50 W GDP allylamine grafting positively impacted MG-63 cell proliferation and increased alkaline phosphatase activity. Gene expression analysis revealed upregulation of OCN, OPG, and COL-I. Both 25 and 50 W GDP allylamine grafting significantly improved zirconia's surface properties (p <.05, p <.01, p <.001). However, only 25 W allylamine grafting with optimal energy settings promoted in vivo osseointegration and new bone formation while preventing bone level loss around the dental implant (p <.05, p <.01, p <.001). Conclusions: This study presents a promising method for enhancing Zr dental implant surface's bioactivity.
KW - allylamine
KW - glow discharge plasma
KW - osseointegration
KW - surface modification
KW - zirconia implants
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U2 - 10.1111/clr.14300
DO - 10.1111/clr.14300
M3 - Article
AN - SCOPUS:85194865568
SN - 0905-7161
VL - 35
SP - 1101
EP - 1113
JO - Clinical Oral Implants Research
JF - Clinical Oral Implants Research
IS - 9
ER -