TY - JOUR
T1 - Alleviation of oxidative damage in multiple tissues in rats with streptozotocin-induced diabetes by rice bran oil supplementation
AU - Hsieh, Rong Hong
AU - Lien, Li Ming
AU - Lin, Shyh Hsiang
AU - Chen, Chia Wen
AU - Cheng, Huei Ju
AU - Cheng, Hsing Hsien
PY - 2005
Y1 - 2005
N2 - The possibility of 8-hydroxy-2′-deoxyguanosine (8-OHdG) serving as a sensitive biomarker of oxidative DNA damage and oxidative stress was investigated. Reactive oxygen species (ROS) have been reported to be a cause of diabetes induced by chemicals such as streptozotocin (STZ) in experimental animals. In this study, we examined oxidative DNA damage in multiple tissues in rats with STZ-induced diabetes by measuring the levels of 8-OHdG in the liver, kidney, pancreas, brain, and heart. Levels of 8-OHdG in mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) were also determined in multiple tissues of rats treated with rice bran oil. Levels were 0.19 ± 0.07, 0.88 ± 0.30, 1.97 ± 0.05, and 9.79 ± 3.09 (1/105 dG) in the liver of nDNA of normal rats, nDNA of STZ-induced diabetic rats, mtDNA of normal rats, and mtDNA of STZ-induced diabetic rats, respectively. Levels of mtDNA of 8-OHdG were 10 times higher than those of nDNA in multiple tissues. Significant reductions in mtDNA 8-OHdG levels were seen in the liver, kidney, and pancreas of diabetic rats treated with rice bran oil compared with diabetic rats without intervention. Our study demonstrated that oxidative mtDNA damage may occur in multiple tissues of STZ-induced diabetics rats. Intervention with rice bran oil treatment may reverse the increase in the frequency of 8-OHdG.
AB - The possibility of 8-hydroxy-2′-deoxyguanosine (8-OHdG) serving as a sensitive biomarker of oxidative DNA damage and oxidative stress was investigated. Reactive oxygen species (ROS) have been reported to be a cause of diabetes induced by chemicals such as streptozotocin (STZ) in experimental animals. In this study, we examined oxidative DNA damage in multiple tissues in rats with STZ-induced diabetes by measuring the levels of 8-OHdG in the liver, kidney, pancreas, brain, and heart. Levels of 8-OHdG in mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) were also determined in multiple tissues of rats treated with rice bran oil. Levels were 0.19 ± 0.07, 0.88 ± 0.30, 1.97 ± 0.05, and 9.79 ± 3.09 (1/105 dG) in the liver of nDNA of normal rats, nDNA of STZ-induced diabetic rats, mtDNA of normal rats, and mtDNA of STZ-induced diabetic rats, respectively. Levels of mtDNA of 8-OHdG were 10 times higher than those of nDNA in multiple tissues. Significant reductions in mtDNA 8-OHdG levels were seen in the liver, kidney, and pancreas of diabetic rats treated with rice bran oil compared with diabetic rats without intervention. Our study demonstrated that oxidative mtDNA damage may occur in multiple tissues of STZ-induced diabetics rats. Intervention with rice bran oil treatment may reverse the increase in the frequency of 8-OHdG.
KW - 8-OHdG
KW - Diabetes
KW - Oxidative stress
KW - Streptozotocin
UR - http://www.scopus.com/inward/record.url?scp=22044440627&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22044440627&partnerID=8YFLogxK
U2 - 10.1196/annals.1338.061
DO - 10.1196/annals.1338.061
M3 - Article
C2 - 15965082
AN - SCOPUS:22044440627
SN - 0077-8923
VL - 1042
SP - 365
EP - 371
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -
