Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis

Michael X. Chen; Ho Hsuan Su; Ching Ya Shiao; Yu Ting Chang; Ming Chu Chang; Chih Chin Kao; San Yuan Wang; Hsi Chang Shih; I. Lin Tsai

doi:10.3390/ijms222111527

Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis

Michael X. Chen, Ho Hsuan Su, Ching Ya Shiao, Yu Ting Chang, Ming Chu Chang, Chih Chin Kao, San Yuan Wang, Hsi Chang Shih, I. Lin Tsai

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.

Original language	English
Article number	11527
Journal	International journal of molecular sciences
Volume	22
Issue number	21
DOIs	https://doi.org/10.3390/ijms222111527
Publication status	Published - Nov 1 2021

Keywords

IgG4
Mass spectrometry
N-glycosylation
Type 1 autoimmune pancreatitis

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/ijms222111527

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Chen, M. X., Su, H. H., Shiao, C. Y., Chang, Y. T., Chang, M. C., Kao, C. C., Wang, S. Y., Shih, H. C., & Tsai, I. L. (2021). Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis. International journal of molecular sciences, 22(21), Article 11527. https://doi.org/10.3390/ijms222111527

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title = "Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis",

abstract = "Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.",

keywords = "IgG4, Mass spectrometry, N-glycosylation, Type 1 autoimmune pancreatitis",

author = "Chen, {Michael X.} and Su, {Ho Hsuan} and Shiao, {Ching Ya} and Chang, {Yu Ting} and Chang, {Ming Chu} and Kao, {Chih Chin} and Wang, {San Yuan} and Shih, {Hsi Chang} and Tsai, {I. Lin}",

note = "Funding Information: Funding: This research and APC was funded by the Ministry of Science and Technology, Taiwan, grant number 107-2314-B-038-019-MY3 and 108-2320-B-038-060-MY3. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Chen, Michael X.

AU - Su, Ho Hsuan

AU - Shiao, Ching Ya

AU - Chang, Yu Ting

AU - Chang, Ming Chu

AU - Kao, Chih Chin

AU - Wang, San Yuan

AU - Shih, Hsi Chang

AU - Tsai, I. Lin

N1 - Funding Information: Funding: This research and APC was funded by the Ministry of Science and Technology, Taiwan, grant number 107-2314-B-038-019-MY3 and 108-2320-B-038-060-MY3. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11/1

Y1 - 2021/11/1

N2 - Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.

AB - Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.

KW - IgG4

KW - Mass spectrometry

KW - N-glycosylation

KW - Type 1 autoimmune pancreatitis

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Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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