Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines

Yi Fen Chiang; Cheng Pei Chung; Jing Hui Lin; Wenchang Chiang; Hsin Yuan Chen; Mohamed Ali; Yin Hwa Shih; Kai Lee Wang; Tsui Chin Huang; Hsin Yi Chang; Li Chun Lin; Tzong Ming Shieh; Shih Min Hsia

doi:10.3390/molecules27133984

Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines

Yi Fen Chiang, Cheng Pei Chung, Jing Hui Lin, Wenchang Chiang, Hsin Yuan Chen, Mohamed Ali, Yin Hwa Shih, Kai Lee Wang, Tsui Chin Huang, Hsin Yi Chang, Li Chun Lin, Tzong Ming Shieh, Shih Min Hsia

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa) cancer cell lines, as well as exploring their possible mechanisms of action. The eth-anolic extracts were obtained from different parts of adlay seed, including AHE (adlay hull extract), ATE (adlay testa extract), ABE (adlay bran extract) and PAE (polished adlay extract). The results of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea showed significant growth inhibitory effects in a dose-dependent manner. The results also showed that the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and decreased CDK4/Cyclin D1 protein expression. Finally, the extract activated caspase-3 activity and PARP protein expression, which induced MCF-7 and HeLa cell apoptosis. We then used liquid chromatography–mass spectrometry (LC/MS) to identify the potential active components., Quercetin showed an anticancer capacity. In conclusion, the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions showed antitumor effects through the inhibition of MCF-7 and HeLa cell line viability, as well as inducing apoptosis and cell cycle arrest.

Original language	English
Article number	3984
Journal	Molecules
Volume	27
Issue number	13
DOIs	https://doi.org/10.3390/molecules27133984
Publication status	Published - Jul 2022

Keywords

adlay seed
apoptosis
breast cancer
caspase-3
cell cycle
cervical cancer

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/molecules27133984

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Chiang, Y. F., Chung, C. P., Lin, J. H., Chiang, W., Chen, H. Y., Ali, M., Shih, Y. H., Wang, K. L., Huang, T. C., Chang, H. Y., Lin, L. C., Shieh, T. M., & Hsia, S. M. (2022). Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines. Molecules, 27(13), Article 3984. https://doi.org/10.3390/molecules27133984

Chiang, YF, Chung, CP, Lin, JH, Chiang, W, Chen, HY, Ali, M, Shih, YH, Wang, KL, Huang, TC, Chang, HY, Lin, LC, Shieh, TM & Hsia, SM 2022, 'Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines', Molecules, vol. 27, no. 13, 3984. https://doi.org/10.3390/molecules27133984

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title = "Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines",

abstract = "The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa) cancer cell lines, as well as exploring their possible mechanisms of action. The eth-anolic extracts were obtained from different parts of adlay seed, including AHE (adlay hull extract), ATE (adlay testa extract), ABE (adlay bran extract) and PAE (polished adlay extract). The results of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea showed significant growth inhibitory effects in a dose-dependent manner. The results also showed that the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and decreased CDK4/Cyclin D1 protein expression. Finally, the extract activated caspase-3 activity and PARP protein expression, which induced MCF-7 and HeLa cell apoptosis. We then used liquid chromatography–mass spectrometry (LC/MS) to identify the potential active components., Quercetin showed an anticancer capacity. In conclusion, the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions showed antitumor effects through the inhibition of MCF-7 and HeLa cell line viability, as well as inducing apoptosis and cell cycle arrest.",

keywords = "adlay seed, apoptosis, breast cancer, caspase-3, cell cycle, cervical cancer",

author = "Chiang, {Yi Fen} and Chung, {Cheng Pei} and Lin, {Jing Hui} and Wenchang Chiang and Chen, {Hsin Yuan} and Mohamed Ali and Shih, {Yin Hwa} and Wang, {Kai Lee} and Huang, {Tsui Chin} and Chang, {Hsin Yi} and Lin, {Li Chun} and Shieh, {Tzong Ming} and Hsia, {Shih Min}",

note = "Funding Information: Funding: This study was supported by grants from the Ministry of Science and Technology, Taiwan (MOST110-2314-B-038-158, MOST110-2628-B-038-018, MOST110-2811-B-038-543, MOST109-2314-B-038-059, MOST109-2628-B-038-015, MOST109-2320-B-254-001 and MOST108-2320-B-214-001). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jul,

doi = "10.3390/molecules27133984",

language = "English",

volume = "27",

journal = "Molecules",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "13",

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TY - JOUR

T1 - Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines

AU - Chiang, Yi Fen

AU - Chung, Cheng Pei

AU - Lin, Jing Hui

AU - Chiang, Wenchang

AU - Chen, Hsin Yuan

AU - Ali, Mohamed

AU - Shih, Yin Hwa

AU - Wang, Kai Lee

AU - Huang, Tsui Chin

AU - Chang, Hsin Yi

AU - Lin, Li Chun

AU - Shieh, Tzong Ming

AU - Hsia, Shih Min

N1 - Funding Information: Funding: This study was supported by grants from the Ministry of Science and Technology, Taiwan (MOST110-2314-B-038-158, MOST110-2628-B-038-018, MOST110-2811-B-038-543, MOST109-2314-B-038-059, MOST109-2628-B-038-015, MOST109-2320-B-254-001 and MOST108-2320-B-214-001). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/7

Y1 - 2022/7

N2 - The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa) cancer cell lines, as well as exploring their possible mechanisms of action. The eth-anolic extracts were obtained from different parts of adlay seed, including AHE (adlay hull extract), ATE (adlay testa extract), ABE (adlay bran extract) and PAE (polished adlay extract). The results of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea showed significant growth inhibitory effects in a dose-dependent manner. The results also showed that the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and decreased CDK4/Cyclin D1 protein expression. Finally, the extract activated caspase-3 activity and PARP protein expression, which induced MCF-7 and HeLa cell apoptosis. We then used liquid chromatography–mass spectrometry (LC/MS) to identify the potential active components., Quercetin showed an anticancer capacity. In conclusion, the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions showed antitumor effects through the inhibition of MCF-7 and HeLa cell line viability, as well as inducing apoptosis and cell cycle arrest.

AB - The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa) cancer cell lines, as well as exploring their possible mechanisms of action. The eth-anolic extracts were obtained from different parts of adlay seed, including AHE (adlay hull extract), ATE (adlay testa extract), ABE (adlay bran extract) and PAE (polished adlay extract). The results of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea showed significant growth inhibitory effects in a dose-dependent manner. The results also showed that the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and decreased CDK4/Cyclin D1 protein expression. Finally, the extract activated caspase-3 activity and PARP protein expression, which induced MCF-7 and HeLa cell apoptosis. We then used liquid chromatography–mass spectrometry (LC/MS) to identify the potential active components., Quercetin showed an anticancer capacity. In conclusion, the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions showed antitumor effects through the inhibition of MCF-7 and HeLa cell line viability, as well as inducing apoptosis and cell cycle arrest.

KW - adlay seed

KW - apoptosis

KW - breast cancer

KW - caspase-3

KW - cell cycle

KW - cervical cancer

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DO - 10.3390/molecules27133984

M3 - Article

C2 - 35807238

AN - SCOPUS:85132987726

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

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ER -

Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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