Adjuvant effect of liposome in chicken result from induction of nitric oxide

Yuh Feng Lin; Ming Chung Deng; Li Ping Tseng; Pei Rong Jiang; Tong Rong Jan; Fang I. Hsieh; Der Zen Liu

doi:10.1088/1748-6041/6/1/015011

Adjuvant effect of liposome in chicken result from induction of nitric oxide

Yuh Feng Lin, Ming Chung Deng, Li Ping Tseng, Pei Rong Jiang, Tong Rong Jan, Fang I. Hsieh, Der Zen Liu

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Intranasal delivery of liposome-encapsulated inactivated Newcastle Disease virus (NDV) is known to be an effective vaccine for inducing immunity in the respiratory tract from our previous reports. Four-week-old specific pathogen-free chickens were intranasally immunized with NDV entrapped in phosphatidylcholine-liposomes (PC-Lip). The mucosal levels of anti-NDV s-immunoglobulin A (IgA), serum IgG, a high hemagglutination inhibition titer (1:640), and the high survival rate with the PC-Lip vaccine were comparable to those of our previous report. The immune mechanisms of the PC-Lip adjuvant were determined by in vitro cellular experiments using the NO production of chicken spleen macrophages. The most important finding of this study was proving that macrophages were stimulated by PC-Lip via the extracellular regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB activation pathways. This finding may be useful for developing potent mucosal vaccine delivery systems in the future.

Original language	English
Article number	015011
Journal	Biomedical Materials
Volume	6
Issue number	1
DOIs	https://doi.org/10.1088/1748-6041/6/1/015011
Publication status	Published - Feb 2011

ASJC Scopus subject areas

Bioengineering
Biomedical Engineering
Biomaterials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1088/1748-6041/6/1/015011

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title = "Adjuvant effect of liposome in chicken result from induction of nitric oxide",

abstract = "Intranasal delivery of liposome-encapsulated inactivated Newcastle Disease virus (NDV) is known to be an effective vaccine for inducing immunity in the respiratory tract from our previous reports. Four-week-old specific pathogen-free chickens were intranasally immunized with NDV entrapped in phosphatidylcholine-liposomes (PC-Lip). The mucosal levels of anti-NDV s-immunoglobulin A (IgA), serum IgG, a high hemagglutination inhibition titer (1:640), and the high survival rate with the PC-Lip vaccine were comparable to those of our previous report. The immune mechanisms of the PC-Lip adjuvant were determined by in vitro cellular experiments using the NO production of chicken spleen macrophages. The most important finding of this study was proving that macrophages were stimulated by PC-Lip via the extracellular regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB activation pathways. This finding may be useful for developing potent mucosal vaccine delivery systems in the future.",

author = "Lin, {Yuh Feng} and Deng, {Ming Chung} and Tseng, {Li Ping} and Jiang, {Pei Rong} and Jan, {Tong Rong} and Hsieh, {Fang I.} and Liu, {Der Zen}",

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AU - Lin, Yuh Feng

AU - Deng, Ming Chung

AU - Tseng, Li Ping

AU - Jiang, Pei Rong

AU - Jan, Tong Rong

AU - Hsieh, Fang I.

AU - Liu, Der Zen

PY - 2011/2

Y1 - 2011/2

N2 - Intranasal delivery of liposome-encapsulated inactivated Newcastle Disease virus (NDV) is known to be an effective vaccine for inducing immunity in the respiratory tract from our previous reports. Four-week-old specific pathogen-free chickens were intranasally immunized with NDV entrapped in phosphatidylcholine-liposomes (PC-Lip). The mucosal levels of anti-NDV s-immunoglobulin A (IgA), serum IgG, a high hemagglutination inhibition titer (1:640), and the high survival rate with the PC-Lip vaccine were comparable to those of our previous report. The immune mechanisms of the PC-Lip adjuvant were determined by in vitro cellular experiments using the NO production of chicken spleen macrophages. The most important finding of this study was proving that macrophages were stimulated by PC-Lip via the extracellular regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB activation pathways. This finding may be useful for developing potent mucosal vaccine delivery systems in the future.

AB - Intranasal delivery of liposome-encapsulated inactivated Newcastle Disease virus (NDV) is known to be an effective vaccine for inducing immunity in the respiratory tract from our previous reports. Four-week-old specific pathogen-free chickens were intranasally immunized with NDV entrapped in phosphatidylcholine-liposomes (PC-Lip). The mucosal levels of anti-NDV s-immunoglobulin A (IgA), serum IgG, a high hemagglutination inhibition titer (1:640), and the high survival rate with the PC-Lip vaccine were comparable to those of our previous report. The immune mechanisms of the PC-Lip adjuvant were determined by in vitro cellular experiments using the NO production of chicken spleen macrophages. The most important finding of this study was proving that macrophages were stimulated by PC-Lip via the extracellular regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB activation pathways. This finding may be useful for developing potent mucosal vaccine delivery systems in the future.

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Adjuvant effect of liposome in chicken result from induction of nitric oxide

Abstract

ASJC Scopus subject areas

UN SDGs

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