Additive effect of interleukin-12 and interleukin-18 on the T-helper cell pathway of malignant pleural effusion

N. S. Yao, Y. M. Chen, R. P. Perng, J. Whang-Peng

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Interleukin-18 (IL-18) is a novel cytokine with interferon-γ (IFN-γ)-inducing activity, thus favoring the T-helper type 1 (Th-1) pathway. The present study attempts to define the role of IL-18 on the functions of lymphocytes isolated from malignant pleural effusions (EAL, effusion-associated lymphocytes). EAL from 10 patients with malignant pleural effusion were incubated with IL-2, IL-12, or IL-18 with/without a αCD3 antibody. ELISA, proliferation, and cytotoxicity assays were performed. IL-18 alone was found to have no significant effect on EAL in terms of cytokine production, lymphocyte proliferation, or cytotoxicity against tumor targets. IL-18 also had no significant additive or synergistic effect on IL-2, IL-12, or αCD3 co-cultured EAL. However, when IL-18 was used with IL-12, the highest IFN-γ/IL-10 ratio was derived, suggesting that these two cytokines had an additive effect in leading EAL from the Th-2 to the Th-1 pathway. Furthermore, 1 of 5 patients' EAL had its strongest cytolytic activity against an autologous tumor when the EAL was cultured with IL-2 plus IL-18, as compared with the other 4 patients whose EAL cytolytic activity against autologous tumor was highest when using IL-2 plus αCD3. These findings suggest that IL-18 alone did not have a significant effect on EAL, and that IL-18 did not enhance αCD3's activity on EAL. However, its additive effect with IL-12 in the Th-1 pathway and with IL-2 in its cytolytic activity against an autologous tumor deserve further studies.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
Issue number1
Publication statusPublished - Dec 1 2002
Externally publishedYes


  • Interferon-γ
  • Interleukin-12
  • Interleukin-18
  • Interleukin-2
  • Malignant effusion

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine


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