Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties

Wen Chi Hou; Yaw Huei Lin

doi:10.1002/elps.1150190212

Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties

Wen Chi Hou
, Yaw Huei Lin

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57: on a 15 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.

Original language	English
Pages (from-to)	212-214
Number of pages	3
Journal	Electrophoresis
Volume	19
Issue number	2
DOIs	https://doi.org/10.1002/elps.1150190212
Publication status	Published - Feb 1998
Externally published	Yes

Keywords

Activity staining
Ipomoea batatas (L) Lam
Leaves
Sodium dodecyl sulfate -polyacrylamide gel electrophoresis
Trypsin inhibitors

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.1150190212

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title = "Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties",

abstract = "The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57: on a 15 \% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.",

keywords = "Activity staining, Ipomoea batatas (L) Lam, Leaves, Sodium dodecyl sulfate -polyacrylamide gel electrophoresis, Trypsin inhibitors",

author = "Hou, \{Wen Chi\} and Lin, \{Yaw Huei\}",

year = "1998",

month = feb,

doi = "10.1002/elps.1150190212",

language = "English",

volume = "19",

pages = "212--214",

journal = "Electrophoresis",

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publisher = "John Wiley and Sons Inc.",

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AU - Hou, Wen Chi

AU - Lin, Yaw Huei

PY - 1998/2

Y1 - 1998/2

N2 - The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57: on a 15 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.

AB - The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57: on a 15 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.

KW - Activity staining

KW - Ipomoea batatas (L) Lam

KW - Leaves

KW - Sodium dodecyl sulfate -polyacrylamide gel electrophoresis

KW - Trypsin inhibitors

UR - https://www.scopus.com/pages/publications/0031936391

UR - https://www.scopus.com/pages/publications/0031936391#tab=citedBy

U2 - 10.1002/elps.1150190212

DO - 10.1002/elps.1150190212

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JO - Electrophoresis

JF - Electrophoresis

IS - 2

ER -

Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties

Abstract

Keywords

ASJC Scopus subject areas

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