Activation of topoisomerase II-mediated excision of chromosomal DNA loops during oxidative stress

Tsai Kun Li, Allen Y. Chen, Chiang Yu, Yong Mao, Huimin Wang, Leroy F. Liu

Research output: Contribution to journalArticlepeer-review

151 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2), a reactive oxygen species (ROS), is known to induce oxidative stress and apoptosis. U937 cells treated with H2O2 were shown to produce high molecular weight (HMW) DNA fragments ~50 to 100 kb in size in <1 min. The formation of these HMW DNA fragments is reversible and shown to be mediated by DNA topoisomerase II (TOP2). Following this initial event, formation of irreversible HMW DNA fragments and nucleosomal ladders occurs. Our results thus demonstrate a potential role of TOP2 in oxidative damage of DNA and apoptotic cell death.

Original languageEnglish
Pages (from-to)1553-1560
Number of pages8
JournalGenes and Development
Volume13
Issue number12
DOIs
Publication statusPublished - Jun 15 1999
Externally publishedYes

Keywords

  • Apoptosis
  • HO
  • Oxidative stress
  • TOP2
  • VM-26

ASJC Scopus subject areas

  • General Medicine

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