TY - JOUR
T1 - Activation of the cellular oncogene c-erbB by ltr insertion
T2 - Molecular basis for induction of erythroblastosis by avian leukosis virus
AU - Fung, Yuen Kai T
AU - Lewis, Wynne G.
AU - Crittenden, Lyman B.
AU - Kung, Hsing Jien
PY - 1983/1/1
Y1 - 1983/1/1
N2 - Avian leukosis virus (ALV), a slowly oncogenic retrovirus, induces in chickens a variety of neoplasms, including lymphoid leukosis and erythroblastosis. In lymphoid leukosis, a cellular oncogene, c-myc, is activated by the insertion of ALV LTR. We provide evidence that ALV utilizes a similar mechanism in erythroblastosis induction by activating a different cellular oncogene, c-erbB. We report the isolation, from leukemic erythroblast DNA, of a clone that represents the viral-cell junction fragment and carried the ALV LTR and part of the c-erbB locus. Restriction and sequence analyses reveal that the LTR is located upstream from the erbB coding region and is oriented in the same transcriptional direction; such a structure would be compatible with the promoter-insertion type of activation. Our findings provide a molecular explanation for the multipotency of slowly oncogenic retroviruses.
AB - Avian leukosis virus (ALV), a slowly oncogenic retrovirus, induces in chickens a variety of neoplasms, including lymphoid leukosis and erythroblastosis. In lymphoid leukosis, a cellular oncogene, c-myc, is activated by the insertion of ALV LTR. We provide evidence that ALV utilizes a similar mechanism in erythroblastosis induction by activating a different cellular oncogene, c-erbB. We report the isolation, from leukemic erythroblast DNA, of a clone that represents the viral-cell junction fragment and carried the ALV LTR and part of the c-erbB locus. Restriction and sequence analyses reveal that the LTR is located upstream from the erbB coding region and is oriented in the same transcriptional direction; such a structure would be compatible with the promoter-insertion type of activation. Our findings provide a molecular explanation for the multipotency of slowly oncogenic retroviruses.
UR - http://www.scopus.com/inward/record.url?scp=0020967595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020967595&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(83)90417-8
DO - 10.1016/0092-8674(83)90417-8
M3 - Article
C2 - 6305505
AN - SCOPUS:0020967595
SN - 0092-8674
VL - 33
SP - 357
EP - 368
JO - Cell
JF - Cell
IS - 2
ER -