Acceleration of wound healing in diabetic rats by layered hydrogel dressing

Yen Hsien Lee; Jung Jhih Chang; Ming Chien Yang; Chiang Ting Chien; Wen Fu Lai

doi:10.1016/j.carbpol.2011.12.045

Acceleration of wound healing in diabetic rats by layered hydrogel dressing

Yen Hsien Lee, Jung Jhih Chang, Ming Chien Yang, Chiang Ting Chien, Wen Fu Lai

Graduate Institute of Medical Sciences

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

In this study, a novel layered hydrogel composing of alginate (AL), chitosan (CS), and poly(γ-glutamic acid) (PGA) was prepared. The resulting hydrogel was characterized, including the swelling ratio, water vapor transmission rate, the release of Ca ²⁺ and blood coagulation activity. In vitro evaluation of cell migration and proliferation were carried out on electric cell-substrate impedance sensing (ECIS). Effect of the hydrogels on wound healing was examined in type 1 diabetic rat model induced by streptozotoxin (STZ). After grafting to full-thickness wounds in diabetic rats, AL-CS-PGA exhibited higher rate of wound healing than conventional AL hydrogels. Epithelialization and collagen deposition were examined histologically. Hydroxyproline levels also were assessed in the wound skin. The results indicated AL-CS-PGA treated wounds showed increased collagen regeneration and epithelialization. Therefore AL-CS-PGA can improve wound healing of diabetic rat models comparing to conventional AL wound dressing.

Original language	English
Pages (from-to)	809-819
Number of pages	11
Journal	Carbohydrate Polymers
Volume	88
Issue number	3
DOIs	https://doi.org/10.1016/j.carbpol.2011.12.045
Publication status	Published - Apr 15 2012

Keywords

Alginate
Chitosan
Poly(γ-glutamic acid)
Rat model
Wound healing

ASJC Scopus subject areas

Organic Chemistry
Materials Chemistry
Polymers and Plastics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.carbpol.2011.12.045

Cite this

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title = "Acceleration of wound healing in diabetic rats by layered hydrogel dressing",

abstract = "In this study, a novel layered hydrogel composing of alginate (AL), chitosan (CS), and poly(γ-glutamic acid) (PGA) was prepared. The resulting hydrogel was characterized, including the swelling ratio, water vapor transmission rate, the release of Ca 2+ and blood coagulation activity. In vitro evaluation of cell migration and proliferation were carried out on electric cell-substrate impedance sensing (ECIS). Effect of the hydrogels on wound healing was examined in type 1 diabetic rat model induced by streptozotoxin (STZ). After grafting to full-thickness wounds in diabetic rats, AL-CS-PGA exhibited higher rate of wound healing than conventional AL hydrogels. Epithelialization and collagen deposition were examined histologically. Hydroxyproline levels also were assessed in the wound skin. The results indicated AL-CS-PGA treated wounds showed increased collagen regeneration and epithelialization. Therefore AL-CS-PGA can improve wound healing of diabetic rat models comparing to conventional AL wound dressing.",

keywords = "Alginate, Chitosan, Poly(γ-glutamic acid), Rat model, Wound healing",

author = "Lee, {Yen Hsien} and Chang, {Jung Jhih} and Yang, {Ming Chien} and Chien, {Chiang Ting} and Lai, {Wen Fu}",

year = "2012",

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doi = "10.1016/j.carbpol.2011.12.045",

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AU - Lee, Yen Hsien

AU - Chang, Jung Jhih

AU - Yang, Ming Chien

AU - Chien, Chiang Ting

AU - Lai, Wen Fu

PY - 2012/4/15

Y1 - 2012/4/15

N2 - In this study, a novel layered hydrogel composing of alginate (AL), chitosan (CS), and poly(γ-glutamic acid) (PGA) was prepared. The resulting hydrogel was characterized, including the swelling ratio, water vapor transmission rate, the release of Ca 2+ and blood coagulation activity. In vitro evaluation of cell migration and proliferation were carried out on electric cell-substrate impedance sensing (ECIS). Effect of the hydrogels on wound healing was examined in type 1 diabetic rat model induced by streptozotoxin (STZ). After grafting to full-thickness wounds in diabetic rats, AL-CS-PGA exhibited higher rate of wound healing than conventional AL hydrogels. Epithelialization and collagen deposition were examined histologically. Hydroxyproline levels also were assessed in the wound skin. The results indicated AL-CS-PGA treated wounds showed increased collagen regeneration and epithelialization. Therefore AL-CS-PGA can improve wound healing of diabetic rat models comparing to conventional AL wound dressing.

AB - In this study, a novel layered hydrogel composing of alginate (AL), chitosan (CS), and poly(γ-glutamic acid) (PGA) was prepared. The resulting hydrogel was characterized, including the swelling ratio, water vapor transmission rate, the release of Ca 2+ and blood coagulation activity. In vitro evaluation of cell migration and proliferation were carried out on electric cell-substrate impedance sensing (ECIS). Effect of the hydrogels on wound healing was examined in type 1 diabetic rat model induced by streptozotoxin (STZ). After grafting to full-thickness wounds in diabetic rats, AL-CS-PGA exhibited higher rate of wound healing than conventional AL hydrogels. Epithelialization and collagen deposition were examined histologically. Hydroxyproline levels also were assessed in the wound skin. The results indicated AL-CS-PGA treated wounds showed increased collagen regeneration and epithelialization. Therefore AL-CS-PGA can improve wound healing of diabetic rat models comparing to conventional AL wound dressing.

KW - Alginate

KW - Chitosan

KW - Poly(γ-glutamic acid)

KW - Rat model

KW - Wound healing

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JO - Carbohydrate Polymers

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ER -

Acceleration of wound healing in diabetic rats by layered hydrogel dressing

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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