Accelerated bioethanol fermentation by using a novel yeast immobilization technique: Microtube array membrane

Chien Chung Chen, Chien Hui Wu, Jhih Jhong Wu, Chien Chih Chiu, Chien Hsuan Wong, Min Lang Tsai, Hong Ting Victor Lin

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Cell immobilization is a way to isolate or localize intact cells in a certain space and maintain their catalytic activity. Immobilized cells can effectively reduce the negative effects of inhibitors and the processing cost of inoculum preparation for continuous or fed-batch fermentation of microorganisms. In this study, a novel yeast immobilization technique using renewable poly-l-lactic acid (PLLA) microtube array membrane (MTAM) was thoroughly evaluated for bioethanol fermentation. PLLA-MTAM was shown to be stable in 15% (v/v) ethanol solution during shaking cultivation. A yeast encapsulation efficiency of 67-70% was obtained, and the yeasts in MTAMs with greater porosity showed greater bioethanol productivity. The MTAM-immobilized Kluyveromyces marxianus, prepared using in situ and siphon methods, were evaluated using 5% (w/v) glucose fermentation. Improved glucose consumption and bioethanol production were observed in batch bioethanol fermentation. In 7 cycles during repeated-batch fermentation, the immobilized yeasts prepared using the in situ method showed a maximum CEtOH of 24.23 g/L, maximum YP/S of 0.48 g/g, and r PEtOH of 2.69 g/L h. Our data indicated that the PLLA-MTAM immobilized yeasts significantly enhanced bioethanol productivity and was a novel, promising technology for bioethanol fermentation.

Original languageEnglish
Article number10450
Pages (from-to)1509-1515
Number of pages7
JournalProcess Biochemistry
Issue number10
Publication statusPublished - Oct 1 2015


  • Bioethanol
  • Fermentation
  • Kluyveromyces marxianus
  • Microtube array membrane (MTAM)
  • Repeated-batch

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering


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