TY - GEN
T1 - A viscosity and C-reactive protein sensing technique based on Brownian motion measurements by total-internal-reflective-fluorescent velocimetry
AU - Fan, Yu Jui
AU - Liu, Yi Hsing
AU - Wu, Tzu Heng
AU - Sheen, Horn Jiunn
AU - Lin, Shiming
AU - Tsai, Jing Fa
PY - 2010
Y1 - 2010
N2 - In this study, a two-section clinical sample detection on viscosity and bimolecule sensing technique based on measuring the Brownian velocity of nanobeads has been successfully developed. Bare and antibody-coated fluorescent nanobeads were used to detect various viscous solutions and different concentrations of bio-molecular samples, respectively. The Brownian velocities of the nanobeads became slower when the sizes of antibody-coated nanobeads increased during the antibody-antigen interaction process or when the bare nanobeads were seeded in a higher viscosity solution. In order to obtain the characteristics of the Brownian motion of nanobeads in the static liquid, total internal reflection fluorescent velocimetry (TIRFV) was employed. This sensing technique should be useful in clinical diagnosis, bio-molecule judgment, and the study of fluid characteristics.
AB - In this study, a two-section clinical sample detection on viscosity and bimolecule sensing technique based on measuring the Brownian velocity of nanobeads has been successfully developed. Bare and antibody-coated fluorescent nanobeads were used to detect various viscous solutions and different concentrations of bio-molecular samples, respectively. The Brownian velocities of the nanobeads became slower when the sizes of antibody-coated nanobeads increased during the antibody-antigen interaction process or when the bare nanobeads were seeded in a higher viscosity solution. In order to obtain the characteristics of the Brownian motion of nanobeads in the static liquid, total internal reflection fluorescent velocimetry (TIRFV) was employed. This sensing technique should be useful in clinical diagnosis, bio-molecule judgment, and the study of fluid characteristics.
UR - http://www.scopus.com/inward/record.url?scp=79951904973&partnerID=8YFLogxK
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U2 - 10.1109/ICSENS.2010.5690216
DO - 10.1109/ICSENS.2010.5690216
M3 - Conference contribution
AN - SCOPUS:79951904973
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 395
EP - 400
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -