TY - JOUR
T1 - A T cell-binding fragment of fibrinogen can prevent autoimmunity
AU - Takada, Yoshikazu
AU - Ono, Yoko
AU - Sagusa, Jun
AU - Mitsiades, Constantine
AU - Mitsiades, Nicholas
AU - Tsai, Jean
AU - He, Yong
AU - Maningding, Ernest
AU - Coleman, Annie
AU - Ramirez-Maverakis, Dalila
AU - Rodriquez, Raphael
AU - Takada, Yoko
AU - Maverakis, Emanual
PY - 2010/6
Y1 - 2010/6
N2 - The C-terminal domain of the fibrinogen γ chain (γC) has been shown to bind to the integrins αIIbβ3, αMβ2 and αVβ3. It has also been reported that a peptide derived from the αMβ2-binding site of γC can suppress an animal model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). Here we have truncated γC at position 399 to remove the prothrombotic αIIbβ3-binding site. We show that this truncated version of γC, termed γC399tr, can bind to activated T cells. In addition, T cells incubated with γC399tr secreted less IFN-γ when stimulated with antigen and APC; however, cytokine secretion was unaltered when T cells were stimulated non-specifically with a mixture of anti-CD3 and anti-CD28 antibodies. Thus, only antigen-dependent T cell activation is inhibited by γC399tr. When administered intraperitoneally, γC399tr potently inhibited actively induced EAE and reversed ongoing disease. We hypothesize that the ability of γC399tr to inhibit autoreactive immune responses is a result of its ability to bind integrins. This activity was not solely dependent on the αMβ2 integrin-binding site. When polyalanine was substituted for the αMβ2-binding site, the resulting γC390polyA was still able to inhibit EAE. To our knowledge, this is the first demonstration that T cells can bind to fibrin (ogen), an important extracellular matrix protein that is deposited at sites of inflammation. Our results also identify γC399tr as a novel therapeutic molecule.
AB - The C-terminal domain of the fibrinogen γ chain (γC) has been shown to bind to the integrins αIIbβ3, αMβ2 and αVβ3. It has also been reported that a peptide derived from the αMβ2-binding site of γC can suppress an animal model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). Here we have truncated γC at position 399 to remove the prothrombotic αIIbβ3-binding site. We show that this truncated version of γC, termed γC399tr, can bind to activated T cells. In addition, T cells incubated with γC399tr secreted less IFN-γ when stimulated with antigen and APC; however, cytokine secretion was unaltered when T cells were stimulated non-specifically with a mixture of anti-CD3 and anti-CD28 antibodies. Thus, only antigen-dependent T cell activation is inhibited by γC399tr. When administered intraperitoneally, γC399tr potently inhibited actively induced EAE and reversed ongoing disease. We hypothesize that the ability of γC399tr to inhibit autoreactive immune responses is a result of its ability to bind integrins. This activity was not solely dependent on the αMβ2 integrin-binding site. When polyalanine was substituted for the αMβ2-binding site, the resulting γC390polyA was still able to inhibit EAE. To our knowledge, this is the first demonstration that T cells can bind to fibrin (ogen), an important extracellular matrix protein that is deposited at sites of inflammation. Our results also identify γC399tr as a novel therapeutic molecule.
KW - Autoimmunity
KW - Experimental autoimmune encephalomyelitis (EAE)
KW - MOG
KW - Multiple sclerosis
UR - http://www.scopus.com/inward/record.url?scp=77952237981&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952237981&partnerID=8YFLogxK
U2 - 10.1016/j.jaut.2009.11.017
DO - 10.1016/j.jaut.2009.11.017
M3 - Article
C2 - 20036106
AN - SCOPUS:77952237981
SN - 0896-8411
VL - 34
SP - 453
EP - 459
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
IS - 4
ER -