TY - JOUR
T1 - A substance isolated from cornus offlcinalis enhances the motility of human sperm
AU - Jeng, Hellen
AU - Wu, Chao Mei
AU - Su, Shuen Jiing
AU - Chang, Wen Chang
PY - 1997
Y1 - 1997
N2 - The effects of a Chinese herb, Cornus officinalis, on the motility of human sperm was studied. An aqueous extract was prepared from the dried fruits of the herb and used in this study. The crude extract at a final concentration of 0.5 (ig/|J.l in phosphate buffered saline (pH 7.4) increased sperm motility from 25.8 ±7.7% to 42.8 ±10.3% (i.e. 68% increase, n = 7), as determined by the computer-aided-sperm-analysis (CASA) method. The crude extract was fractionated by high-performance liquid chromatography (HPLC) into four fractions: Cl, C2, C3 and C4. Their effects on sperm motility were further studied by CASA. Only the C4 fraction showed substantial stimulatory effects on sperm motility. At a concentration of 5 ng/u.1, C4 increased the sperm motility from 15.7 ±3.8% to 34.5 ±6.4% (i.e. 120% increase, n = 6) by CASA and from 14.9 + 4.3 to 28.5 ±8.1 (i.e. 91% increase, n = 8) by transmembrane migration ratio (TMMR) method. This result suggests that C4 is the active component in Comus officinalis that enhances sperm motility.
AB - The effects of a Chinese herb, Cornus officinalis, on the motility of human sperm was studied. An aqueous extract was prepared from the dried fruits of the herb and used in this study. The crude extract at a final concentration of 0.5 (ig/|J.l in phosphate buffered saline (pH 7.4) increased sperm motility from 25.8 ±7.7% to 42.8 ±10.3% (i.e. 68% increase, n = 7), as determined by the computer-aided-sperm-analysis (CASA) method. The crude extract was fractionated by high-performance liquid chromatography (HPLC) into four fractions: Cl, C2, C3 and C4. Their effects on sperm motility were further studied by CASA. Only the C4 fraction showed substantial stimulatory effects on sperm motility. At a concentration of 5 ng/u.1, C4 increased the sperm motility from 15.7 ±3.8% to 34.5 ±6.4% (i.e. 120% increase, n = 6) by CASA and from 14.9 + 4.3 to 28.5 ±8.1 (i.e. 91% increase, n = 8) by transmembrane migration ratio (TMMR) method. This result suggests that C4 is the active component in Comus officinalis that enhances sperm motility.
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U2 - 10.1142/s0192415x97000330
DO - 10.1142/s0192415x97000330
M3 - Article
C2 - 9358903
AN - SCOPUS:0031307308
SN - 0192-415X
VL - 25
SP - 301
EP - 306
JO - American Journal of Chinese Medicine
JF - American Journal of Chinese Medicine
IS - 3-4
ER -