Abstract
Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 481-483 |
| Number of pages | 3 |
| Journal | American Journal of Clinical Pathology |
| Volume | 79 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 1983 |
| Externally published | Yes |
ASJC Scopus subject areas
- Pathology and Forensic Medicine