Abstract
A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and γ-dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
Original language | English |
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Pages (from-to) | 777-784 |
Number of pages | 8 |
Journal | Combinatorial Chemistry and High Throughput Screening |
Volume | 9 |
Issue number | 10 |
DOIs | |
Publication status | Published - Dec 2006 |
Keywords
- Flow cytometry
- High-throughput screening
- Human umbilical cord blood mononuclear cells
- Immuno-regulatory activities
- Natural products
- γ-dodecalactone
ASJC Scopus subject areas
- Drug Discovery
- Computer Science Applications
- Organic Chemistry