TY - JOUR
T1 - A Sandwich-Type Electrochemical Immunosensor for Insulin Detection Based on Au-Adhered Cu5Zn8 Hollow Porous Carbon Nanocubes and AuNP Deposited Nitrogen-Doped Holey Graphene
AU - Sakthivel, Rajalakshmi
AU - Prasanna, Sanjay Ballur
AU - Tseng, Ching Li
AU - Lin, Lu Yin
AU - Duann, Yeh Fang
AU - He, Jr Hau
AU - Chung, Ren Jei
N1 - Funding Information:
The authors are grateful for the financial supports of this research by the Ministry of Science and Technology of Taiwan (MOST 106‐2221‐E‐027‐034 and MOST 109‐2222‐E‐027‐004), and the International Distinguished Visiting Professor support for Prof. J.‐H.H. from National Taipei University of Technology. Technical assistance from the Precision Analysis and Material Research Center of National Taipei University of Technology (Taipei Tech) is appreciated. Technical assistance from Prof. Hsieh‐Chih Tsai of National Taiwan University of Science and Technology with the Raman spectroscopy is appreciated.
Funding Information:
The authors are grateful for the financial supports of this research by the Ministry of Science and Technology of Taiwan (MOST 106-2221-E-027-034 and MOST 109-2222-E-027-004), and the International Distinguished Visiting Professor support for Prof. J.-H.H. from National Taipei University of Technology. Technical assistance from the Precision Analysis and Material Research Center of National Taipei University of Technology (Taipei Tech) is appreciated. Technical assistance from Prof. Hsieh-Chih Tsai of National Taiwan University of Science and Technology with the Raman spectroscopy is appreciated.
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/9
Y1 - 2022/9
N2 - Rapid, accurate, and sensitive insulin detection is crucial for managing and treating diabetes. A simple sandwich-type electrochemical immunosensor is engineered using gold nanoparticle (AuNP)-adhered metal–organic framework-derived copper–zinc hollow porous carbon nanocubes (Au@Cu5Zn8/HPCNC) and AuNP-deposited nitrogen-doped holey graphene (NHG) are used as a dual functional label and sensing platform. The results show that identical morphology and size of Au@Cu5Zn8/HPCNC enhance the electrocatalytic active sites, conductivity, and surface area to immobilize the detection antibodies (Ab2). In addition, AuNP/NHG has the requisite biocompatibility and electrical conductivity, which facilitates electron transport and increases the surface area of the capture antibody (Ab1). Significantly, Cu5Zn8/HPCNC exhibits necessary catalytic activity and sensitivity for the electrochemical reduction of H2O2 using (i–t) amperometry and improves the electrochemical response in differential pulse voltammetry. Under optimal conditions, the immunosensor for insulin demonstrates a wide linear range with a low detection limit and viable specificity, stability, and reproducibility. The platform's practicality is evaluated by detecting insulin in human serum samples. All these characteristics indicate that the Cu5Zn8/HPCNC-based biosensing strategy may be used for the point-of-care assay of diverse biomarkers.
AB - Rapid, accurate, and sensitive insulin detection is crucial for managing and treating diabetes. A simple sandwich-type electrochemical immunosensor is engineered using gold nanoparticle (AuNP)-adhered metal–organic framework-derived copper–zinc hollow porous carbon nanocubes (Au@Cu5Zn8/HPCNC) and AuNP-deposited nitrogen-doped holey graphene (NHG) are used as a dual functional label and sensing platform. The results show that identical morphology and size of Au@Cu5Zn8/HPCNC enhance the electrocatalytic active sites, conductivity, and surface area to immobilize the detection antibodies (Ab2). In addition, AuNP/NHG has the requisite biocompatibility and electrical conductivity, which facilitates electron transport and increases the surface area of the capture antibody (Ab1). Significantly, Cu5Zn8/HPCNC exhibits necessary catalytic activity and sensitivity for the electrochemical reduction of H2O2 using (i–t) amperometry and improves the electrochemical response in differential pulse voltammetry. Under optimal conditions, the immunosensor for insulin demonstrates a wide linear range with a low detection limit and viable specificity, stability, and reproducibility. The platform's practicality is evaluated by detecting insulin in human serum samples. All these characteristics indicate that the Cu5Zn8/HPCNC-based biosensing strategy may be used for the point-of-care assay of diverse biomarkers.
KW - Cu Zn hollow porous carbon nanocube
KW - insulin
KW - nitrogen-doped holey graphene
KW - sandwich-type electrochemical immunosensor
KW - sensing platform
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U2 - 10.1002/smll.202202516
DO - 10.1002/smll.202202516
M3 - Article
C2 - 35950565
AN - SCOPUS:85135803070
SN - 1613-6810
VL - 18
JO - Small
JF - Small
IS - 35
M1 - 2202516
ER -