A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao; Chiu Min Cheng; Kuo Hsiang Chuang; Chih Hung Chuang; Shey Cherng Tzou; Ta Chun Cheng; Yuan Chin Hsieh; Kuang Wen Liao; Yun Ming Wang; Long Sen Chang; Steve R. Roffler; Fang Ming Chen; Tian Lu Cheng

doi:10.1016/j.ab.2012.08.001

A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao
, Chiu Min Cheng
, Kuo Hsiang Chuang
, Chih Hung Chuang
, Shey Cherng Tzou
, Ta Chun Cheng
, Yuan Chin Hsieh
, Kuang Wen Liao
, Yun Ming Wang
, Long Sen Chang
, Steve R. Roffler
, Fang Ming Chen
, Tian Lu Cheng

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R²= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Analytical Biochemistry
Volume	431
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2012.08.001
Publication status	Published - Dec 1 2012

Keywords

14-Epitope tag protein ladder
Anti-epitope tag antibodies
Epitope tags
Protein molecular estimation
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Western blot analysis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.ab.2012.08.001

Cite this

@article{75869fc2b65a4d5cb7bf450806a7432c,

title = "A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis",

abstract = "We designed a protein ladder (hereafter referred to as {"}Mega- tag{"}) that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R2= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.",

keywords = "14-Epitope tag protein ladder, Anti-epitope tag antibodies, Epitope tags, Protein molecular estimation, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot analysis",

author = "Kao, \{Chien Han\} and Cheng, \{Chiu Min\} and Chuang, \{Kuo Hsiang\} and Chuang, \{Chih Hung\} and Tzou, \{Shey Cherng\} and Cheng, \{Ta Chun\} and Hsieh, \{Yuan Chin\} and Liao, \{Kuang Wen\} and Wang, \{Yun Ming\} and Chang, \{Long Sen\} and Roffler, \{Steve R.\} and Chen, \{Fang Ming\} and Cheng, \{Tian Lu\}",

note = "Funding Information: This work was supported by grants from the National Research Program for Biopharmaceuticals, National Science Council, Taipei, Taiwan (NSC 100-2325-B-037-001 and 100-2321-B-037-006), the Department of Health, Executive Yuan, Taiwan (DOH100-TD-N-111-010 and DOH100-TD-C-111-002), and the Grant of Biosignature in Colorectal Cancers, Academia Sinica, Taiwan, and Kaohsiung Medical University Research Foundation (KMUER014 and KMUER012). ",

year = "2012",

month = dec,

day = "1",

doi = "10.1016/j.ab.2012.08.001",

language = "English",

volume = "431",

pages = "1--3",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

AU - Kao, Chien Han

AU - Cheng, Chiu Min

AU - Chuang, Kuo Hsiang

AU - Chuang, Chih Hung

AU - Tzou, Shey Cherng

AU - Cheng, Ta Chun

AU - Hsieh, Yuan Chin

AU - Liao, Kuang Wen

AU - Wang, Yun Ming

AU - Chang, Long Sen

AU - Roffler, Steve R.

AU - Chen, Fang Ming

AU - Cheng, Tian Lu

N1 - Funding Information: This work was supported by grants from the National Research Program for Biopharmaceuticals, National Science Council, Taipei, Taiwan (NSC 100-2325-B-037-001 and 100-2321-B-037-006), the Department of Health, Executive Yuan, Taiwan (DOH100-TD-N-111-010 and DOH100-TD-C-111-002), and the Grant of Biosignature in Colorectal Cancers, Academia Sinica, Taiwan, and Kaohsiung Medical University Research Foundation (KMUER014 and KMUER012).

PY - 2012/12/1

Y1 - 2012/12/1

N2 - We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R2= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

AB - We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R2= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

KW - 14-Epitope tag protein ladder

KW - Anti-epitope tag antibodies

KW - Epitope tags

KW - Protein molecular estimation

KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

KW - Western blot analysis

UR - https://www.scopus.com/pages/publications/84867698112

UR - https://www.scopus.com/pages/publications/84867698112#tab=citedBy

U2 - 10.1016/j.ab.2012.08.001

DO - 10.1016/j.ab.2012.08.001

M3 - Article

C2 - 22885722

AN - SCOPUS:84867698112

SN - 0003-2697

VL - 431

SP - 1

EP - 3

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this