A quantitative immunosensing technique based on the measurement of nanobeads' Brownian motion

Yu Jui Fan; Horn Jiunn Sheen; Chia Jui Hsu; Cheng Pang Liu; Shiming Lin; Kuang Chong Wu

doi:10.1016/j.bios.2009.07.037

A quantitative immunosensing technique based on the measurement of nanobeads' Brownian motion

Yu Jui Fan, Horn Jiunn Sheen, Chia Jui Hsu, Cheng Pang Liu, Shiming Lin, Kuang Chong Wu

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A novel bio-sensing technique based on the measurement of nanobeads' Brownian motion using a micro-particle tracking velocimetry (micro-PTV) has been successfully developed to detect antigen-antibody interactions. The rapid interaction between antigens (C-reactive proteins, CRPs) and nanobeads with conjugated antibodies (anti-CRPs) has enabled real-time detection of CRPs to be easily carried out. During the binding process of CRPs to nanobeads, the mean value of the beads' diameters increases so that the Brownian velocity decreases with the increase of time. Moreover, higher CRP concentration leads to a lower Brownian velocity of the nanobeads in the equilibrium state. From the results, the limit of detection 0.1 μg/ml was observed and could be further improved by using smaller nanobeads. Moreover, based on kinetic analysis, the average dissociation constant K_D = (6.48 ± 1.43) × 10^-7 found by this technique for anti-CRP was shown to be in close agreement with the literature values obtained by dual-polarization interferometry (DPI) and indirect competition enzyme-linked immunosorbent assay (ELISA). This simple sensing method can further be used to detect other bio-molecules and viruses.

Original language	English
Pages (from-to)	688-694
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/j.bios.2009.07.037
Publication status	Published - Dec 15 2009
Externally published	Yes

Keywords

Bio-sensing technique
Brownian motion
C-reactive protein
Kinetic analysis
Micro-PTV

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2009.07.037

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title = "A quantitative immunosensing technique based on the measurement of nanobeads' Brownian motion",

abstract = "A novel bio-sensing technique based on the measurement of nanobeads' Brownian motion using a micro-particle tracking velocimetry (micro-PTV) has been successfully developed to detect antigen-antibody interactions. The rapid interaction between antigens (C-reactive proteins, CRPs) and nanobeads with conjugated antibodies (anti-CRPs) has enabled real-time detection of CRPs to be easily carried out. During the binding process of CRPs to nanobeads, the mean value of the beads' diameters increases so that the Brownian velocity decreases with the increase of time. Moreover, higher CRP concentration leads to a lower Brownian velocity of the nanobeads in the equilibrium state. From the results, the limit of detection 0.1 μg/ml was observed and could be further improved by using smaller nanobeads. Moreover, based on kinetic analysis, the average dissociation constant KD = (6.48 ± 1.43) × 10-7 found by this technique for anti-CRP was shown to be in close agreement with the literature values obtained by dual-polarization interferometry (DPI) and indirect competition enzyme-linked immunosorbent assay (ELISA). This simple sensing method can further be used to detect other bio-molecules and viruses.",

keywords = "Bio-sensing technique, Brownian motion, C-reactive protein, Kinetic analysis, Micro-PTV",

author = "Fan, {Yu Jui} and Sheen, {Horn Jiunn} and Hsu, {Chia Jui} and Liu, {Cheng Pang} and Shiming Lin and Wu, {Kuang Chong}",

note = "Funding Information: This work was supported by National Science Council of Taiwan ( NSC 95-2120-M-002-006 and NSC 96-2221-E-002-199 ). ",

year = "2009",

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doi = "10.1016/j.bios.2009.07.037",

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AU - Fan, Yu Jui

AU - Sheen, Horn Jiunn

AU - Hsu, Chia Jui

AU - Liu, Cheng Pang

AU - Lin, Shiming

AU - Wu, Kuang Chong

N1 - Funding Information: This work was supported by National Science Council of Taiwan ( NSC 95-2120-M-002-006 and NSC 96-2221-E-002-199 ).

PY - 2009/12/15

Y1 - 2009/12/15

N2 - A novel bio-sensing technique based on the measurement of nanobeads' Brownian motion using a micro-particle tracking velocimetry (micro-PTV) has been successfully developed to detect antigen-antibody interactions. The rapid interaction between antigens (C-reactive proteins, CRPs) and nanobeads with conjugated antibodies (anti-CRPs) has enabled real-time detection of CRPs to be easily carried out. During the binding process of CRPs to nanobeads, the mean value of the beads' diameters increases so that the Brownian velocity decreases with the increase of time. Moreover, higher CRP concentration leads to a lower Brownian velocity of the nanobeads in the equilibrium state. From the results, the limit of detection 0.1 μg/ml was observed and could be further improved by using smaller nanobeads. Moreover, based on kinetic analysis, the average dissociation constant KD = (6.48 ± 1.43) × 10-7 found by this technique for anti-CRP was shown to be in close agreement with the literature values obtained by dual-polarization interferometry (DPI) and indirect competition enzyme-linked immunosorbent assay (ELISA). This simple sensing method can further be used to detect other bio-molecules and viruses.

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A quantitative immunosensing technique based on the measurement of nanobeads' Brownian motion

Abstract

Keywords

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