TY - JOUR
T1 - A pilot study for circadian gene disturbance in dementia patients
AU - Liu, Hsing Cheng
AU - Hu, Chaur Jong
AU - Tang, Yun Chin
AU - Chang, Jan Gowth
N1 - Funding Information:
The manuscript has been critical reviewed and edited by Su Guo and Chian-Jue Kuo for statistic analysis. This study was supported in part by grants from the National Science Council, Taiwan (NSC 93-2321-B-196-001), Topnotch Stroke Research Center Grant, Ministry of Education, Taiwan and an internal grant from the China Medical University, Taichung, Taiwan (CMU-93-M-21).
PY - 2008/4/25
Y1 - 2008/4/25
N2 - Disturbance of circadian gene regulation might contribute to behavioral and psychological symptoms in dementia patients. This study was to evaluate the CpG island methylation status on the circadian gene promoters in dementia patients. We conducted a set of methylation specific polymerase chain reaction (mPCR) followed by nucleotide sequencing to analyze the methylation status within the promoters of nine circadian-related genes, including PER1, PER2, PER3, CRY1, CRY2, CLOCK, BMAL1, TIM and CK1ε, in the genomic DNA from the peripheral blood leukocytes of 80 dementia patients and 80 age- and gender-matched controls. A total of seven dementia patients (7/80) had CpG island methylation in the circadian genes and none of the controls had methylation. There were three and four patients had CpG island methylation on the promoters of PER1 and CRY1, respectively. Dementia with Lewy body (DLB) patients had the significantly highest frequency of circadian gene CpG island methylation (35.7%). It suggested that epigenetic methylation of circadian gene was more prevalent in dementia patients, especially for the DLB patients. The significance of circadian gene methylation in clinical behavior/sleep disturbance in dementia patients needs further study.
AB - Disturbance of circadian gene regulation might contribute to behavioral and psychological symptoms in dementia patients. This study was to evaluate the CpG island methylation status on the circadian gene promoters in dementia patients. We conducted a set of methylation specific polymerase chain reaction (mPCR) followed by nucleotide sequencing to analyze the methylation status within the promoters of nine circadian-related genes, including PER1, PER2, PER3, CRY1, CRY2, CLOCK, BMAL1, TIM and CK1ε, in the genomic DNA from the peripheral blood leukocytes of 80 dementia patients and 80 age- and gender-matched controls. A total of seven dementia patients (7/80) had CpG island methylation in the circadian genes and none of the controls had methylation. There were three and four patients had CpG island methylation on the promoters of PER1 and CRY1, respectively. Dementia with Lewy body (DLB) patients had the significantly highest frequency of circadian gene CpG island methylation (35.7%). It suggested that epigenetic methylation of circadian gene was more prevalent in dementia patients, especially for the DLB patients. The significance of circadian gene methylation in clinical behavior/sleep disturbance in dementia patients needs further study.
KW - Alzheimer's disease
KW - Circadian rhythm
KW - Dementia with Lewy body
KW - Epigenetic
KW - Methylation
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U2 - 10.1016/j.neulet.2008.02.041
DO - 10.1016/j.neulet.2008.02.041
M3 - Article
C2 - 18358604
AN - SCOPUS:41549090375
SN - 0304-3940
VL - 435
SP - 229
EP - 233
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -