@article{8b9b1b2187b642199f925009fdf363f3,
title = "A Pilot Safety Assessment for Recombinant Epinephelus lanceolatus Piscidin Yeast Powder as a Drug Food Additive after Subacute and Subchronic Administration to SD Rats",
abstract = "Recombinant Epinephelus lanceolatus piscidin (RELP) was previously shown to improve growth performance and immune response when used as a feed additive for Gallus gallus domesticus. However, the long-term toxicity of RELP has not be thoroughly investigated. In the present study, we evaluated the subacute and subchronic oral toxicities of RELP in SD rats by hematological, biochemical, and histopathological analyses. To determine subacute and subchronic toxicities, male and female rats were fed with RELP 1000 mg/kg bodyweight/day for 28 and 90 days, respectively. Bodyweight and food intake were unchanged by RELP treatment over the course of the studies. After exposure, samples of blood, heart, lung, liver, and kidney were collected and analyzed. Results demonstrated that RELP exposure did not cause any observable hematological, biochemical, or histological abnormalities in SD rats. Thus, RELP may be a safe feed additive for use in agriculture and aquaculture.",
keywords = "recombinant Epinephelus lanceolatus piscidin, safety assessment, SD rats, subacute toxicity, subchronic toxicity, yeast powder",
author = "Su, {Bor Chyuan} and Li, {Chao Chin} and Liu, {Chia Wen} and Chen, {Jyh Yih}",
note = "Funding Information: All research funds were obtained from the Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaushi, Ilan, Taiwan to Jyh-Yih Chen. Research money from the Council of Agriculture, Executive Yuan, Taiwan (R.O.C.) supported the study from Jan 2018 to Dec 2020 (the application of antimicrobial peptides as feed additives for fowls). This research was funded by Taipei Medical University, grant number: TMU108-AE1-B22. Funding Information: Funding: All research funds were obtained from the Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaushi, Ilan, Taiwan to Jyh-Yih Chen. Research money from the Council of Agriculture, Executive Yuan, Taiwan (R.O.C.) supported the study from Jan 2018 to Dec 2020 (the application of antimicrobial peptides as feed additives for fowls). This research was funded by Taipei Medical University, grant number: TMU108-AE1-B22. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = nov,
day = "24",
doi = "10.3390/md18120586",
language = "English",
volume = "18",
journal = "Marine Drugs",
issn = "1660-3397",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",
}