A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Shih Hwa Chiou; Chung Lan Kao; Chi Hsien Peng; Shih Jen Chen; Yih Wen Tarng; Hung Hai Ku; Yu Chih Chen; Yi Ming Shyr; Ren Shyan Liu; Chien Jen Hsu; De Ming Yang; Wen Ming Hsu; Cheng Deng Kuo; Chen Hsen Lee

doi:10.1016/j.bbrc.2004.11.061

A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Shih Hwa Chiou, Chung Lan Kao, Chi Hsien Peng, Shih Jen Chen, Yih Wen Tarng, Hung Hai Ku, Yu Chih Chen, Yi Ming Shyr, Ren Shyan Liu, Chien Jen Hsu, De Ming Yang, Wen Ming Hsu, Cheng Deng Kuo, Chen Hsen Lee

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

Human retinal pigment epithelium (HRPE) cells are important in maintaining the normal physiology within the neurosensory retina and photoreceptors. Recently, transplantation of HRPE has become a possible therapeutic approach for retinal degeneration. By negative immunoselection (CD45 and glycophorin A), in this study, we have isolated and cultivated adult human bone marrow stem cells (BMSCs) with multilineage differentiation potential. After a 2- to 4-week culture under chondrogenic, osteogenic, adipogenic, and hepatogenic induction medium, these BMSCs were found to differentiate into cartilage, bone, adipocyte, and hepatocyte-like cells, respectively. We also showed that these BMSCs could differentiate into neural precursor cells (nestin-positive) and mature neurons (MAP-2 and Tuj1-positive) following treatment of neural selection and induction medium for 1 month. Furthermore, the plasticity of BMSCs was confirmed by initiating their differentiation into retinal cells and photoreceptor lineages by co-culturing with HRPE cells. The latter system provides an ex vivo expansion model of culturing photoreceptors for the treatment of retinal degeneration diseases.

Original language	English
Pages (from-to)	578-585
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	326
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.11.061
Publication status	Published - Jan 21 2005
Externally published	Yes

Keywords

Human bone marrow stem cell
Human retinal pigment epithelium
Photoreceptor
Retinal differentiation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2004.11.061

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Chiou, S. H., Kao, C. L., Peng, C. H., Chen, S. J., Tarng, Y. W., Ku, H. H., Chen, Y. C., Shyr, Y. M., Liu, R. S., Hsu, C. J., Yang, D. M., Hsu, W. M., Kuo, C. D., & Lee, C. H. (2005). A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells. Biochemical and Biophysical Research Communications, 326(3), 578-585. https://doi.org/10.1016/j.bbrc.2004.11.061

Chiou, SH, Kao, CL, Peng, CH, Chen, SJ, Tarng, YW, Ku, HH, Chen, YC, Shyr, YM, Liu, RS, Hsu, CJ, Yang, DM, Hsu, WM, Kuo, CD & Lee, CH 2005, 'A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells', Biochemical and Biophysical Research Communications, vol. 326, no. 3, pp. 578-585. https://doi.org/10.1016/j.bbrc.2004.11.061

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abstract = "Human retinal pigment epithelium (HRPE) cells are important in maintaining the normal physiology within the neurosensory retina and photoreceptors. Recently, transplantation of HRPE has become a possible therapeutic approach for retinal degeneration. By negative immunoselection (CD45 and glycophorin A), in this study, we have isolated and cultivated adult human bone marrow stem cells (BMSCs) with multilineage differentiation potential. After a 2- to 4-week culture under chondrogenic, osteogenic, adipogenic, and hepatogenic induction medium, these BMSCs were found to differentiate into cartilage, bone, adipocyte, and hepatocyte-like cells, respectively. We also showed that these BMSCs could differentiate into neural precursor cells (nestin-positive) and mature neurons (MAP-2 and Tuj1-positive) following treatment of neural selection and induction medium for 1 month. Furthermore, the plasticity of BMSCs was confirmed by initiating their differentiation into retinal cells and photoreceptor lineages by co-culturing with HRPE cells. The latter system provides an ex vivo expansion model of culturing photoreceptors for the treatment of retinal degeneration diseases.",

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AU - Chen, Shih Jen

AU - Tarng, Yih Wen

AU - Ku, Hung Hai

AU - Chen, Yu Chih

AU - Shyr, Yi Ming

AU - Liu, Ren Shyan

AU - Hsu, Chien Jen

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AU - Hsu, Wen Ming

AU - Kuo, Cheng Deng

AU - Lee, Chen Hsen

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AB - Human retinal pigment epithelium (HRPE) cells are important in maintaining the normal physiology within the neurosensory retina and photoreceptors. Recently, transplantation of HRPE has become a possible therapeutic approach for retinal degeneration. By negative immunoselection (CD45 and glycophorin A), in this study, we have isolated and cultivated adult human bone marrow stem cells (BMSCs) with multilineage differentiation potential. After a 2- to 4-week culture under chondrogenic, osteogenic, adipogenic, and hepatogenic induction medium, these BMSCs were found to differentiate into cartilage, bone, adipocyte, and hepatocyte-like cells, respectively. We also showed that these BMSCs could differentiate into neural precursor cells (nestin-positive) and mature neurons (MAP-2 and Tuj1-positive) following treatment of neural selection and induction medium for 1 month. Furthermore, the plasticity of BMSCs was confirmed by initiating their differentiation into retinal cells and photoreceptor lineages by co-culturing with HRPE cells. The latter system provides an ex vivo expansion model of culturing photoreceptors for the treatment of retinal degeneration diseases.

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A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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