A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen; Ching Yu Lin; Cheng Chun Kuan; Hsien Yi Sung; Ching San Chen

doi:10.1021/jf020527q

A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen, Ching Yu Lin, Cheng Chun Kuan, Hsien Yi Sung, Ching San Chen

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.

Original language	English
Pages (from-to)	7258-7263
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	50
Issue number	25
DOIs	https://doi.org/10.1021/jf020527q
Publication status	Published - Dec 4 2002
Externally published	Yes

Keywords

Bruchid resistance
Defensin
In vitro translation
Mungbean

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/jf020527q

Cite this

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title = "A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid",

abstract = "A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.",

keywords = "Bruchid resistance, Defensin, In vitro translation, Mungbean",

author = "Chen, {Kuan Chung} and Lin, {Ching Yu} and Kuan, {Cheng Chun} and Sung, {Hsien Yi} and Chen, {Ching San}",

year = "2002",

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doi = "10.1021/jf020527q",

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TY - JOUR

T1 - A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

AU - Chen, Kuan Chung

AU - Lin, Ching Yu

AU - Kuan, Cheng Chun

AU - Sung, Hsien Yi

AU - Chen, Ching San

PY - 2002/12/4

Y1 - 2002/12/4

N2 - A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.

AB - A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.

KW - Bruchid resistance

KW - Defensin

KW - In vitro translation

KW - Mungbean

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JO - Journal of Agricultural and Food Chemistry

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IS - 25

ER -

A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Abstract

Keywords

ASJC Scopus subject areas

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