Recently, the study of in vitro capillary network using microfluidic devices by generating single concentration gradient have uncovered many biological information of angiogenesis. However, the platform for angiogenesis inhibitors have not been proposed. In this study, we have developed a microfluidic device for generation of multi gradients to stimuli cancer-induced angiogenesis and fibroblast-induced angiogenesis simultaneously. The device features an interaction chamber, connected to the surrounding channels with radian shaped low-height structures used to locate clusters of cells. The formation of multiple gradients is achieved in the interaction chamber through diffusion and sustained by continuous perfusion. Microfluidic valve was developed to control the direction of flow for trapping cells of different types respectively. We show that our device could generate multiple concentration gradient and trap clusters of cells. These results demonstrated that our device is able to stimuli the angiogenesis and can be used to do the angiogenesis inhibitor study.
|Title of host publication
|2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
|Institute of Electrical and Electronics Engineers Inc.
|Number of pages
|Published - Aug 5 2015
|18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015 → Jun 25 2015
|18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
|6/21/15 → 6/25/15
- cell manipulation
- gradient generator
- in vitro model
ASJC Scopus subject areas
- Electrical and Electronic Engineering