A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching

Chun Hui Sung; Yao Fei Chan; Kang Yun Lee; Sheng Ming Wu; Cheng Hsien Liu

doi:10.1109/NEMS.2018.8556978

A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching

Chun Hui Sung, Yao Fei Chan, Kang Yun Lee, Sheng Ming Wu, Cheng Hsien Liu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Asthma is a disease about lower respiratory tract inflammation. A global survey shows that there are about 230 million people with asthma. Asthma is an important issue of public health in the developed and developing countries. We propose a stretchable microfluidic bronchial chip made by polydimethylsiloxane (PDMS) and glass for studying the effects of cell stretching in asthma in vitro. The bronchial smooth muscle cells (BSMC) were cultured on microfluidic chip under a continuous stretching and release mimicking human breathing. Stretching device consists of 3D printer components, a servo motor and a control panel. We have demonstrated that regular stretching of cells affect its properties. We performed stretching of cells with 13% elongation in 15 cycle per minute for 2 days. The stretching affected cell alignment of bronchial smooth muscle cells. We observed that direction of cell growth and cell stretching direction are perpendicular to each other.

Original language	English
Title of host publication	NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	36-39
Number of pages	4
ISBN (Electronic)	9781538652732
DOIs	https://doi.org/10.1109/NEMS.2018.8556978
Publication status	Published - Dec 3 2018
Event	13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018 - Singapore, Singapore Duration: Apr 22 2018 → Apr 26 2018

Publication series

Name	NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Conference

Conference	13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018
Country/Territory	Singapore
City	Singapore
Period	4/22/18 → 4/26/18

Keywords

bronchial muscle cells
Cell stretching
Microfluidics

ASJC Scopus subject areas

Biomedical Engineering
Electrical and Electronic Engineering
Instrumentation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/NEMS.2018.8556978

Cite this

Sung, C. H., Chan, Y. F., Lee, K. Y., Wu, S. M., & Liu, C. H. (2018). A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching. In NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 36-39). Article 8556978 (NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2018.8556978

A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching. / Sung, Chun Hui; Chan, Yao Fei; Lee, Kang Yun et al.
NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Institute of Electrical and Electronics Engineers Inc., 2018. p. 36-39 8556978 (NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sung, CH, Chan, YF, Lee, KY, Wu, SM & Liu, CH 2018, A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching. in NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 8556978, NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, Institute of Electrical and Electronics Engineers Inc., pp. 36-39, 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018, Singapore, Singapore, 4/22/18. https://doi.org/10.1109/NEMS.2018.8556978

Sung CH, Chan YF, Lee KY, Wu SM, Liu CH. A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching. In NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Institute of Electrical and Electronics Engineers Inc. 2018. p. 36-39. 8556978. (NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems). doi: 10.1109/NEMS.2018.8556978

Sung, Chun Hui ; Chan, Yao Fei ; Lee, Kang Yun et al. / A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching. NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 36-39 (NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

@inproceedings{ea6309c6676b4605bbfb5c7ebcd53a4a,

title = "A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching",

abstract = "Asthma is a disease about lower respiratory tract inflammation. A global survey shows that there are about 230 million people with asthma. Asthma is an important issue of public health in the developed and developing countries. We propose a stretchable microfluidic bronchial chip made by polydimethylsiloxane (PDMS) and glass for studying the effects of cell stretching in asthma in vitro. The bronchial smooth muscle cells (BSMC) were cultured on microfluidic chip under a continuous stretching and release mimicking human breathing. Stretching device consists of 3D printer components, a servo motor and a control panel. We have demonstrated that regular stretching of cells affect its properties. We performed stretching of cells with 13% elongation in 15 cycle per minute for 2 days. The stretching affected cell alignment of bronchial smooth muscle cells. We observed that direction of cell growth and cell stretching direction are perpendicular to each other.",

keywords = "bronchial muscle cells, Cell stretching, Microfluidics",

author = "Sung, {Chun Hui} and Chan, {Yao Fei} and Lee, {Kang Yun} and Wu, {Sheng Ming} and Liu, {Cheng Hsien}",

note = "Publisher Copyright: {\textcopyright} 2018 IEEE.; 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018 ; Conference date: 22-04-2018 Through 26-04-2018",

year = "2018",

month = dec,

day = "3",

doi = "10.1109/NEMS.2018.8556978",

language = "English",

series = "NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "36--39",

booktitle = "NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

address = "United States",

}

TY - GEN

T1 - A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching

AU - Sung, Chun Hui

AU - Chan, Yao Fei

AU - Lee, Kang Yun

AU - Wu, Sheng Ming

AU - Liu, Cheng Hsien

PY - 2018/12/3

Y1 - 2018/12/3

N2 - Asthma is a disease about lower respiratory tract inflammation. A global survey shows that there are about 230 million people with asthma. Asthma is an important issue of public health in the developed and developing countries. We propose a stretchable microfluidic bronchial chip made by polydimethylsiloxane (PDMS) and glass for studying the effects of cell stretching in asthma in vitro. The bronchial smooth muscle cells (BSMC) were cultured on microfluidic chip under a continuous stretching and release mimicking human breathing. Stretching device consists of 3D printer components, a servo motor and a control panel. We have demonstrated that regular stretching of cells affect its properties. We performed stretching of cells with 13% elongation in 15 cycle per minute for 2 days. The stretching affected cell alignment of bronchial smooth muscle cells. We observed that direction of cell growth and cell stretching direction are perpendicular to each other.

AB - Asthma is a disease about lower respiratory tract inflammation. A global survey shows that there are about 230 million people with asthma. Asthma is an important issue of public health in the developed and developing countries. We propose a stretchable microfluidic bronchial chip made by polydimethylsiloxane (PDMS) and glass for studying the effects of cell stretching in asthma in vitro. The bronchial smooth muscle cells (BSMC) were cultured on microfluidic chip under a continuous stretching and release mimicking human breathing. Stretching device consists of 3D printer components, a servo motor and a control panel. We have demonstrated that regular stretching of cells affect its properties. We performed stretching of cells with 13% elongation in 15 cycle per minute for 2 days. The stretching affected cell alignment of bronchial smooth muscle cells. We observed that direction of cell growth and cell stretching direction are perpendicular to each other.

KW - bronchial muscle cells

KW - Cell stretching

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=85060295492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060295492&partnerID=8YFLogxK

U2 - 10.1109/NEMS.2018.8556978

DO - 10.1109/NEMS.2018.8556978

M3 - Conference contribution

AN - SCOPUS:85060295492

T3 - NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

SP - 36

EP - 39

BT - NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018

Y2 - 22 April 2018 through 26 April 2018

ER -

A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this