TY - JOUR
T1 - A metagenomics study of hexabromocyclododecane degradation with a soil microbial community
AU - Li, Yi Jie
AU - Chuang, Chia Hsien
AU - Cheng, Wen Chih
AU - Chen, Shu Hwa
AU - Chen, Wen Ling
AU - Lin, Yu Jie
AU - Lin, Chung Yen
AU - Shih, Yang hsin
N1 - Funding Information:
This work was supported by the Grant from Ministry of Science and Technology , Taiwan ( 106-2221-E-002 -043 -MY3 ), and the Fund from National Taiwan University and Academia Sinica , Taiwan ( NTUAS-110L04312 and NTUAS-111L04312 ). support this study. We also thank the National Health Research Institutes (NHRI) in Taiwan for performing the Nanopore sequencing experiments.
Funding Information:
This work was supported by the Grant from Ministry of Science and Technology, Taiwan (106-2221-E-002 -043 -MY3), and the Fund from National Taiwan University and Academia Sinica, Taiwan (NTUAS-110L04312 and NTUAS-111L04312). support this study. We also thank the National Health Research Institutes (NHRI) in Taiwan for performing the Nanopore sequencing experiments.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/5/15
Y1 - 2022/5/15
N2 - Hexabromocyclododecanes (HBCDs) are globally prevalent and persistent organic pollutants (POPs) listed by the Stockholm Convention in 2013. They have been detected in many environmental media from waterbodies to Plantae and even in the human body. Due to their highly bioaccumulative characterization, they pose an urgent public health issue. Here, we demonstrate that the indigenous microbial community in the agricultural soil in Taiwan could decompose HBCDs with no additional carbon source incentive. The degradation kinetics reached 0.173 day-1 after the first treatment and 0.104 day-1 after second exposure. With additional C-sources, the rate constants decreased to 0.054–0.097 day-1. The hydroxylic debromination metabolites and ring cleavage long-chain alkane metabolites were identified to support the potential metabolic pathways utilized by the soil microbial communities. The metagenome established by Nanopore sequencing showed significant compositional alteration in the soil microbial community after the HBCD treatment. After ranking, comparing relative abundances, and performing network analyses, several novel bacterial taxa were identified to contribute to HBCD biotransformation, including Herbaspirillum, Sphingomonas, Brevundimonas, Azospirillum, Caulobacter, and Microvirga, through halogenated / aromatic compound degradation, glutathione-S-transferase, and hydrolase activity. We present a compelling and applicable approach combining metagenomics research, degradation kinetics, and metabolomics strategies, which allowed us to decipher the natural attenuation and remediation mechanisms of HBCDs.
AB - Hexabromocyclododecanes (HBCDs) are globally prevalent and persistent organic pollutants (POPs) listed by the Stockholm Convention in 2013. They have been detected in many environmental media from waterbodies to Plantae and even in the human body. Due to their highly bioaccumulative characterization, they pose an urgent public health issue. Here, we demonstrate that the indigenous microbial community in the agricultural soil in Taiwan could decompose HBCDs with no additional carbon source incentive. The degradation kinetics reached 0.173 day-1 after the first treatment and 0.104 day-1 after second exposure. With additional C-sources, the rate constants decreased to 0.054–0.097 day-1. The hydroxylic debromination metabolites and ring cleavage long-chain alkane metabolites were identified to support the potential metabolic pathways utilized by the soil microbial communities. The metagenome established by Nanopore sequencing showed significant compositional alteration in the soil microbial community after the HBCD treatment. After ranking, comparing relative abundances, and performing network analyses, several novel bacterial taxa were identified to contribute to HBCD biotransformation, including Herbaspirillum, Sphingomonas, Brevundimonas, Azospirillum, Caulobacter, and Microvirga, through halogenated / aromatic compound degradation, glutathione-S-transferase, and hydrolase activity. We present a compelling and applicable approach combining metagenomics research, degradation kinetics, and metabolomics strategies, which allowed us to decipher the natural attenuation and remediation mechanisms of HBCDs.
KW - Biodegradation
KW - Hexabromocyclododecane
KW - Metagenomics
KW - Microbial network analysis
KW - Nanopore sequencing
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U2 - 10.1016/j.jhazmat.2022.128465
DO - 10.1016/j.jhazmat.2022.128465
M3 - Article
AN - SCOPUS:85124821373
SN - 0304-3894
VL - 430
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 128465
ER -