A crystallographic investigation of citrate synthase from pig and chicken heart muscle

Andrew H.J. Wang; M. L. Sherman; Alexander Rich

doi:10.1016/0006-291X(78)90589-2

A crystallographic investigation of citrate synthase from pig and chicken heart muscle

Andrew H.J. Wang, M. L. Sherman, Alexander Rich

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P4₁2₁2 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P2₁2₁2 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.

Original language	English
Pages (from-to)	150-156
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(78)90589-2
Publication status	Published - May 15 1978
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(78)90589-2

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@article{5216e6e5dda54057a043d10a8839e52f,

title = "A crystallographic investigation of citrate synthase from pig and chicken heart muscle",

abstract = "Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P41212 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P21212 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 {\AA} between these two crystal forms, while b- and c-axis dimensions are virtually identical.",

author = "Wang, {Andrew H.J.} and Sherman, {M. L.} and Alexander Rich",

note = "Funding Information: We thank Dr. Chi-Yu Lee (National Institute of Environmental Health Sciences) for providing the 8-(6-aminohexyl)-amino-5{\textquoteright}-ATP ligand and his helpful discussion. The research was supported by grants from the N. I. H., N.S.F., N.A.S.A., and the American Cancer Society. A. H. -J. Wang was partially supported by the Center for Cancer Research at M. I. T. on Grant No. CA 14051 of the N. I. H.",

year = "1978",

month = may,

day = "15",

doi = "10.1016/0006-291X(78)90589-2",

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pages = "150--156",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - A crystallographic investigation of citrate synthase from pig and chicken heart muscle

AU - Wang, Andrew H.J.

AU - Sherman, M. L.

AU - Rich, Alexander

N1 - Funding Information: We thank Dr. Chi-Yu Lee (National Institute of Environmental Health Sciences) for providing the 8-(6-aminohexyl)-amino-5’-ATP ligand and his helpful discussion. The research was supported by grants from the N. I. H., N.S.F., N.A.S.A., and the American Cancer Society. A. H. -J. Wang was partially supported by the Center for Cancer Research at M. I. T. on Grant No. CA 14051 of the N. I. H.

PY - 1978/5/15

Y1 - 1978/5/15

N2 - Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P41212 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P21212 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.

AB - Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P41212 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P21212 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.

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DO - 10.1016/0006-291X(78)90589-2

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AN - SCOPUS:0017893276

SN - 0006-291X

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EP - 156

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

A crystallographic investigation of citrate synthase from pig and chicken heart muscle

Abstract

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