A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

Yi Chung Chien; Ming Jyh Sheu; Chieh Hsi Wu; Wen Hsin Lin; Ying Yi Chen; Po Liang Cheng; Hsu Chen Cheng

doi:10.1186/1472-6882-12-137

A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

Yi Chung Chien, Ming Jyh Sheu, Chieh Hsi Wu, Wen Hsin Lin, Ying Yi Chen, Po Liang Cheng, Hsu Chen Cheng

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Original language	English
Article number	1082
Journal	BMC Complementary and Alternative Medicine
Volume	12
DOIs	https://doi.org/10.1186/1472-6882-12-137
Publication status	Published - Aug 24 2012
Externally published	Yes

Keywords

Chinese herbs
Gan-Lu-Yin
Migration
Restenosis
Vascular smooth muscle cell

ASJC Scopus subject areas

Complementary and alternative medicine

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10.1186/1472-6882-12-137

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Chien, Y. C., Sheu, M. J., Wu, C. H., Lin, W. H., Chen, Y. Y., Cheng, P. L., & Cheng, H. C. (2012). A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways. BMC Complementary and Alternative Medicine, 12, Article 1082. https://doi.org/10.1186/1472-6882-12-137

A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways. / Chien, Yi Chung; Sheu, Ming Jyh; Wu, Chieh Hsi et al.
In: BMC Complementary and Alternative Medicine, Vol. 12, 1082, 24.08.2012.

Research output: Contribution to journal › Article › peer-review

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title = "A Chinese herbal formula {"}Gan-Lu-Yin{"} suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways",

abstract = "Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.",

keywords = "Chinese herbs, Gan-Lu-Yin, Migration, Restenosis, Vascular smooth muscle cell",

author = "Chien, {Yi Chung} and Sheu, {Ming Jyh} and Wu, {Chieh Hsi} and Lin, {Wen Hsin} and Chen, {Ying Yi} and Cheng, {Po Liang} and Cheng, {Hsu Chen}",

note = "Funding Information: We are grateful to Jeffery Conrad for English writing assistance. The study was supported by grants from the National Science Council (NSC 100-2320-B-039-014-), China Medical University (CMU99-TC-29), and Department of Health Clinical Trial and Research Center of Excellence (DOH101-TD-B-111-004 and DOH101-TD-B-111-005). This research was also supported by the National Science Council and by the Ministry of Education, Taiwan, Republic of China, under the ATU plan (NSC 101-2311-B-005-006-MY3).",

year = "2012",

month = aug,

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doi = "10.1186/1472-6882-12-137",

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AU - Chien, Yi Chung

AU - Sheu, Ming Jyh

AU - Wu, Chieh Hsi

AU - Lin, Wen Hsin

AU - Chen, Ying Yi

AU - Cheng, Po Liang

AU - Cheng, Hsu Chen

N1 - Funding Information: We are grateful to Jeffery Conrad for English writing assistance. The study was supported by grants from the National Science Council (NSC 100-2320-B-039-014-), China Medical University (CMU99-TC-29), and Department of Health Clinical Trial and Research Center of Excellence (DOH101-TD-B-111-004 and DOH101-TD-B-111-005). This research was also supported by the National Science Council and by the Ministry of Education, Taiwan, Republic of China, under the ATU plan (NSC 101-2311-B-005-006-MY3).

PY - 2012/8/24

Y1 - 2012/8/24

N2 - Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

AB - Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

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KW - Migration

KW - Restenosis

KW - Vascular smooth muscle cell

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A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

Abstract

Keywords

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