A candidate gene for Drosophila genome methylation

M. Narsa Reddy; Lin Ya Tang; Tai Lin Lee; C. K.James Shen

doi:10.1038/sj.onc.1206650

A candidate gene for Drosophila genome methylation

M. Narsa Reddy, Lin Ya Tang, Tai Lin Lee, C. K.James Shen

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).

Original language	English
Pages (from-to)	6301-6303
Number of pages	3
Journal	Oncogene
Volume	22
Issue number	40
DOIs	https://doi.org/10.1038/sj.onc.1206650
Publication status	Published - Sept 18 2003
Externally published	Yes

Keywords

DNA methylation
Drosophila cells
Enzyme

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/sj.onc.1206650

Cite this

@article{d5e7f20442ce4681a4695ad9b5400764,

title = "A candidate gene for Drosophila genome methylation",

abstract = "Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).",

keywords = "DNA methylation, Drosophila cells, Enzyme",

author = "Reddy, {M. Narsa} and Tang, {Lin Ya} and Lee, {Tai Lin} and Shen, {C. K.James}",

year = "2003",

month = sep,

day = "18",

doi = "10.1038/sj.onc.1206650",

language = "English",

volume = "22",

pages = "6301--6303",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Nature Publishing Group",

number = "40",

}

TY - JOUR

T1 - A candidate gene for Drosophila genome methylation

AU - Reddy, M. Narsa

AU - Tang, Lin Ya

AU - Lee, Tai Lin

AU - Shen, C. K.James

PY - 2003/9/18

Y1 - 2003/9/18

N2 - Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).

AB - Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).

KW - DNA methylation

KW - Drosophila cells

KW - Enzyme

UR - http://www.scopus.com/inward/record.url?scp=0142088850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142088850&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1206650

DO - 10.1038/sj.onc.1206650

M3 - Article

C2 - 13679869

AN - SCOPUS:0142088850

SN - 0950-9232

VL - 22

SP - 6301

EP - 6303

JO - Oncogene

JF - Oncogene

IS - 40

ER -

A candidate gene for Drosophila genome methylation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this