981 Alterations of gene expression in papulopustular rosacea by whole transcriptome analysis

Y. Shih; J. Xu; A. Kumar; R. Li; A. Chang

doi:10.1016/j.jid.2019.03.1057

981 Alterations of gene expression in papulopustular rosacea by whole transcriptome analysis

Y. Shih, J. Xu, A. Kumar, R. Li, A. Chang

Taipei Medical University Shuang Ho Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

Rosacea is a common and incurable skin disorder. Identification of key molecular pathways found in rosacea may lead to new and better directions to treat this disease. The main objective was to identify differentially expressed genes in papulopustular rosacea skin in an unbiased fashion by whole transcriptome analysis. After IRB approval and written informed consent, rosacea skin biopsies from 6 individuals with untreated, papulopustular rosacea and their adjacent non-lesional skin were obtained. These samples were subjected to RNA extraction and RNA-sequencing (Illumina HiSeq 4000). Significantly altered genes were identified by pairwise comparison of rosacea versus adjacent non-lesional skin from the same individual by DESeq. Selected gene expression differences were confirmed by qRT-PCR. Gene Ontology (GO) term analysis was performed to identify biologic themes. Analysis identified 487 significantly altered genes (p

Original language	Undefined/Unknown
Pages (from-to)	S170
Journal	Journal of Investigative Dermatology
Volume	139
Issue number	5, Supplement
DOIs	https://doi.org/10.1016/j.jid.2019.03.1057
Publication status	Published - 2019

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10.1016/j.jid.2019.03.1057

https://www.sciencedirect.com/science/article/pii/S0022202X19312485

Cite this

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title = "981 Alterations of gene expression in papulopustular rosacea by whole transcriptome analysis",

abstract = "Rosacea is a common and incurable skin disorder. Identification of key molecular pathways found in rosacea may lead to new and better directions to treat this disease. The main objective was to identify differentially expressed genes in papulopustular rosacea skin in an unbiased fashion by whole transcriptome analysis. After IRB approval and written informed consent, rosacea skin biopsies from 6 individuals with untreated, papulopustular rosacea and their adjacent non-lesional skin were obtained. These samples were subjected to RNA extraction and RNA-sequencing (Illumina HiSeq 4000). Significantly altered genes were identified by pairwise comparison of rosacea versus adjacent non-lesional skin from the same individual by DESeq. Selected gene expression differences were confirmed by qRT-PCR. Gene Ontology (GO) term analysis was performed to identify biologic themes. Analysis identified 487 significantly altered genes (p",

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AU - Shih, Y.

AU - Xu, J.

AU - Kumar, A.

AU - Li, R.

AU - Chang, A.

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Y1 - 2019

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