Abstract
We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.
Original language | English |
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Pages (from-to) | 351-354 |
Number of pages | 4 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 377 |
Issue number | 2 |
DOIs | |
Publication status | Published - Dec 12 2008 |
Keywords
- 9-cis retinoic acid
- Mitochondrial biogenesis
- Retinoid X receptor
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology