This study investigated the effect of intravenous glutamine (GLN) supplementation on the liver's inflammatory response and oxidative stress in a mouse model of polymicrobial sepsis. Mice were randomly assigned to a normal control (NC) group and two sepsis groups. Sepsis was induced by cecal ligation and puncture (CLP). One hour after CLP, septic mice were given saline (SS) or 0.75 g GLN/kg of body weight (SG) once via a tail vein. Septic mice were sacrificed at 24 or 48 h after CLP, and the livers were harvested for further analysis. Results showed that sepsis resulted in higher myeloperoxidase (MPO) activity and malondialdehyde (MDA) production. Interleukin (IU-1, IL-6, and tumor necrosis factor (TNF)-α levels in the liver were upregulated, whereas IL-10 and transforming growth factor (TGF)-β had decreased at 24 and/or 48 h after CLP. Compared to the SS group, the SG group had lower concentrations of IL-1, IL-6, and TNF-α and higher IL-10 and TGF-β levels. Also, MPO activity and MDA concentrations were reduced in liver tissues. These results suggest that sepsis results in inflammation of liver tissues. A single dose of intravenous GLN administration after CLP reduced oxidative stress and attenuated the inflammatory response in livers of mice with polymicrobial sepsis.
|Translated title of the contribution
|Glutamine Modulates Liver Inflammation And Oxidative Stress in Septic Mice
|Number of pages
|Journal of the Chinese Nutrition Society
|Published - Sept 1 2015
- proinflammatory cytokine
- oxidative stress