Abstract
Adlay bran (AB) possesses physiological functions on inhibiting cancer, anti-inflammation, improving metabolic syndromes, and modulation on endocrine. Phenolic compounds, polymethoxyflavones (PMFs), and unique coixspirolactams were regarded as important active components in AB. However, optimized extracting procedure for above ingredients is lacking. In the present study, yield, total polyphenol, and total flavonoids contents in AB ethanolic extract (ABE) were founded to be affected mainly by temperature and concentration of ethanol(aq)' According to response surface methodology (RSM) evaluation, the optimized extracting condition of ABE was under 50°C with 8-folds of 55% ethanol(aq) for 120 min. ABE made by above procedures was analyzed on a high-performance liquid chromatography (HPLC) system; andp-hydroxybenzoic acid (11.86 μ/g), vanillin (6.57 μ/g), and ferulic acid (6.36 μ/g) were relatively abundant in ABE. Moreover, coixspirolactam E and PMFs, including 3,5,7,4'-tetramethoxyfiavone, 3,5,7,3',4'-pentamethoxyfiavone, 3,5,6,7,8,3',4'-heptamethoxyfiavone, tangererin, and nobiletin, were clearly identified by HPLC-mass spectrum (HPLC-MS) platform. The results would be used for product development and quality control of ABE.
| Translated title of the contribution | Optimization on Preparation of Active Extract from Adlay Bran and Qualitative Analysis for Indicative Components |
|---|---|
| Original language | Chinese (Traditional) |
| Pages (from-to) | 153-163 |
| Number of pages | 11 |
| Journal | Taiwanese Journal of Agricultural Chemistry and Food Science |
| Volume | 61 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sept 2023 |
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology
- Agronomy and Crop Science
