細胞在奈米/次微米溝槽表面的行為研究

Directing mammalian cell behavior using biophysical cues such as topography in the extracellular microenvironment is crucial for the successes in the biomedical devices, implants, and tissue engineering. It has been suggested that groove/ridge topography modulate cellular behaviors in different biological level. The goal of this dissertation is to understand the effect of nano/submicron grooved surface on the behaviors of skeletal myoblasts, cardiomyocytes, and rat mesenchymal stem cells (rMSCs). Nano/submicron grooved surfaces affect focal adhesion, cell alignment, and function of cell in a remarkable manner, dominating by the depth of groove. Myogenic index of aligned skeletal myoblasts is enhanced on the grooved surfaces compared to the flat control, due to increase in the end-to-end fusion. Peptide conjugation further enhances the myogenic index and meantime morphologically parallel myotubes. The contractile function of aligned cardiomyocytes is upregulated on the grooved surface compared to the flat control, because the anisotropic morphology facilitates synchronous contraction of cardiomyocytes. The rigidity of substrate also affects the contraction of cardiomyocytes. Grooved surfaces have minor effect on cell proliferation and osteogenesis of rMSCs compared to the flat control, while these surfaces enhance the early myogenesis and adipogenesis of rMSCs. Chemical factor and topographic cue both affect the rMSCs differentiation, while the former plays a more momentous role. Taken together, nano/submicron grooved surface modulated focal adhesion and cell morphology anisotropically, depending on the feature size, which in turn changes the differentiation and functionality of cells in a cell-type dependent manner.