白蛋白接著於丙烯胺電漿處理鈦金屬表面的離體研究

In this study, our goal was to develop a glow-discharge method to functionalize titanium surfaces by the covalent immobilization of bioactive organic molecules and then to evaluate the bioactivity in vitro. The titanium plats were first cleaned by glow discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to free-terminal NH2 groups using glutaraldehyde as a coupling agent. Surface coverage by bound albumin was evaluated by SEM visualization. The results implied that the albumin was successfully grafted on to the titanium plates by the glow discharge technology, and this method offers the possibility of covalently linking selected molecules in order to guide and promote tissue healing that occurs during implant integration in bone and soft tissue. The argon-plasma treatment time was proportional to the surface roughness and degree of cleaning. They revealed that bombardment by energetic radicals and ions had occurred during plasma treatment. This implied that the plasma treatment did not lead to argon damage or a nitridation effect. Allylamine with terminal NH2 groups was successfully linked the with plasma-treated titanium plate. The binding energy shift of the N 1s peak was obvious. This revealed that allylamine was ionized by the plasma treatments, and that the dangling bond acted as a medium to link with albumin. Osteoblast-like MC3T3-E1 cells were cultured on the plasma-treated titanium plates for from 8 to 48 hours. Results showed that the osteoblast-like cells spread radically after 8 hours, and better adhesion were observed after 24 hours. This implied that the plasma-treated titanium surface possessed better bioactivity and biocompatibility than that without plasma modification.