Senile dementia is an important aging problem, including Alzheimer’s disease and Parkinson’s disease which occur as neurodegenerative processes. People have to pay attention to this topic due to aging population and current trend of increased Alzheimer's disease patients worldwide. So far the causal agent of senile dementia is still unknown, but we may reduce the incidence of Alzheimer’s disease and the neurodegeneration rate through increasing neuroprotection and suppressing neuronal inflammation and apoptosis. Recent study reveals that several ingredients of the extracts isolated from Antrodia cinnamomea (AC) mycelia possess complicated pharmacological and biological activities, which can decrease the production of reactive oxygen species (ROS). In addition, the extracts from AC fruiting body with anti-inflammatory effect can inhibit ROS production and decrease attached ability of neutrophils. Previously, researchers isolate a new compound, 24-Methylen elanosta-7, 9(11)-diene-3 , 15 -diol-21-oicacid, designated as MMH01, from the fruiting body of AC, and found that possesses toxicity against human leukemia and pancreatic cancer cells, even up to 97% in anti-cancer effect. Therefore, this study used human neuroblastoma cell line, IMR-32 to detect whether AC could enhance the neural cell activity. Most of neural cells are growth arrest or growth slowness; however we found that MMH01 with an appropriate-dose manner was able to increase the healthy cells ratio and raise the activity of antioxidant enzyme. We also found that the expressions of inflammatory genes like IL-1β, IL-6, iNOS et al are dose-dependently correlated with the concentration of MMH01, while CCL2 may play a vital role in regulation of neural cells activity. Further study of the mechanisms of AC action is needed to define how MMH01 contribute to increase IMR-32 cell activity.
|Translated title of the contribution
|Evaluation of Antrodia cinnamomea Extract MMH01 Effect on IMR-32 Neural Cells Activity
|Number of pages
|Published - 2015