Schistosoma japonicum is a zoonotic trematode, which is endemic in Indonesia, Philippines and mainland China. The most serious country is mainland China. Schistosomiasis japonica causes pathological lesions in several organs. Morbidity is primarily due to deposition and entrapment of parasite eggs within host tissues which induce a delayed hypersensitivity granulomatous responses, leading to gross enlargement of the liver, spleen and intestine, fibrosis, and eventually hepatic destruction, cirrhosis and liver failure in chronic infection. This study develops 26KDa Glutathione S-transferase recombinant protein by using RT-PCR technology, and uses this antigen as immunodiagnostic reagent for human schistosomiasis japonica. The results showed that the O.D. values of serum samples from acute and chronic patients were statistically significant higher than the O.D. values of serum samples from healthy peoples in endemic and non-endemic areas. No immune cross reactions were found in acute and chronic patients compared with the patients infected with Paragonimus westermani, hookworms and Ascaris lumbricoides. These results indicated that this recombinant 26kDa GST might be a good immunodiagnostic antigen for human schistosomiasis japonica.
|Translated title of the contribution
|An Evaluation of Recombinant 26 KDa Glutathione S-transferase of Schistosoma Japonicum as an Antigen for Serodiagnosis in Human Schistosomiasis Japonica
|Published - 2007
- Schistosomiasis Japonica
- Immunodiagnostic reagent
- Glutathione S-transferase
- Specificity and sensitivity