先進雷射石墨烯結構製程技術於生物分子元件應用之研究

陳 肇祈, 張 天立

Research output: Types of ThesisMaster's Thesis

Abstract

The realization of multi-functional biomedical sensors for human health care, not only to preventing diseases in life, but also predictive to obtain the patient's medical messages. These patients can receive effective medical treatment further at once after the disease and achieve faster healing compared to hospital diagnosis. In this study, the multi-layer graphene (MLG) thin-film based electrode structures were fabricated by pulsed laser ablation (PLA) direct writing for advanced laser processing technology. Among them, the wavelength of ultrafast picosecond pulsed laser (355/532-UPPL), and nanosecond pulsed laser (355-NPL) were used. Then the effects and and characterization of MLG thin films can be investigated. At the same time, the development of microfluidic device design and electrospun nanofibers technology can be applied to detect the different biomolecules.

Based on the laser processing technology to form the on-chip device for glucose detection, the linear I-V curves demostrated that the detection of GOD were obtained by monitoring the change of the electronic characteristics of the MLG thin-film based device. Here, the electrical respond revealed a good linear dependence in the glucose concentration range from 1 to 10 mM. For the application of microfluidic device, the reducing dross on the surface of ablated pillar array channels with the scanning curve process can be used at the optimal parameters in the clockwise direction. The C of glass device can reach the value of 0.04 μm/pulse that depends on the number of pulses applied to the microfluidic process by a simple model. For the application of electrospun nanowires, PVA-G hybrid nanofiber devices that operation was possible at temperatures of up to 60 °C with the minimal power consumption of 265.25 mW at the PVA-G concentration of 6%. When the Relative humidity (RH) was 80%, the humidity detection device indicated that the excellent sensing properties of the electric response, response time, and recovery time were 66.4%, 11 sec, and 35 sec, respectively. For the application of PCR, the HA-RTC PCR device was possibly shown BK virus (BKV) within 60 min where the marker and its VP1 fragment at 354 bp can be performed entirely diagnosis (amplification). The device with formed microheaters with the MLG thin-film electrodes was verified the good temperature retention and thermal conductivity.

Therefore, a single-step process can be integrated to achieve the requirements of mask-less, miniaturization, rapid production and small volume detection in the design and application of biomedical detection. Furthermore, the development potential of graphene based thin-film devices with electrospinning composite nanofibers was demonstrated the on-chip sensing device for biomolecule detection.
Original languageChinese (Traditional)
Awarding Institution
  • 臺灣師範大學機電工程學系學位論文
Publisher
DOIs
Publication statusPublished - Jan 2018
Externally publishedYes

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