κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

Bidita Salahuddin; Mostafa Kamal Masud; Shazed Aziz; Chia Hung Liu; Nasim Amiralian; Aditya Ashok; S. M.Azad Hossain; Hyeongyu Park; Md Abdul Wahab; Mohammed A. Amin; M. Adharvana Chari; Alan E. Rowan; Yusuke Yamauchi; Md Shahriar A. Hossain; Yusuf Valentino Kaneti

doi:10.1246/bcsj.20210286

κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

Bidita Salahuddin, Mostafa Kamal Masud, Shazed Aziz, Chia Hung Liu, Nasim Amiralian, Aditya Ashok, S. M.Azad Hossain, Hyeongyu Park, Md Abdul Wahab, Mohammed A. Amin, M. Adharvana Chari, Alan E. Rowan, Yusuke Yamauchi, Md Shahriar A. Hossain, Yusuf Valentino Kaneti

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs.

Original language	English
Pages (from-to)	198-207
Number of pages	10
Journal	Bulletin of the Chemical Society of Japan
Volume	95
Issue number	1
DOIs	https://doi.org/10.1246/bcsj.20210286
Publication status	Published - 2022

Keywords

Gold electrode
Mesoporous metals
MicroRNA sensor

ASJC Scopus subject areas

General Chemistry

Access to Document

10.1246/bcsj.20210286

Cite this

Salahuddin, B., Masud, M. K., Aziz, S., Liu, C. H., Amiralian, N., Ashok, A., Hossain, S. M. A., Park, H., Wahab, M. A., Amin, M. A., Chari, M. A., Rowan, A. E., Yamauchi, Y., Hossain, M. S. A., & Kaneti, Y. V. (2022). κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA. Bulletin of the Chemical Society of Japan, 95(1), 198-207. https://doi.org/10.1246/bcsj.20210286

Salahuddin, B, Masud, MK, Aziz, S, Liu, CH, Amiralian, N, Ashok, A, Hossain, SMA, Park, H, Wahab, MA, Amin, MA, Chari, MA, Rowan, AE, Yamauchi, Y, Hossain, MSA & Kaneti, YV 2022, 'κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA', Bulletin of the Chemical Society of Japan, vol. 95, no. 1, pp. 198-207. https://doi.org/10.1246/bcsj.20210286

@article{20ece1b2edc24af285d13938ce668914,

title = "κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA",

abstract = "Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs. ",

keywords = "Gold electrode, Mesoporous metals, MicroRNA sensor",

author = "Bidita Salahuddin and Masud, {Mostafa Kamal} and Shazed Aziz and Liu, {Chia Hung} and Nasim Amiralian and Aditya Ashok and Hossain, {S. M.Azad} and Hyeongyu Park and Wahab, {Md Abdul} and Amin, {Mohammed A.} and Chari, {M. Adharvana} and Rowan, {Alan E.} and Yusuke Yamauchi and Hossain, {Md Shahriar A.} and Kaneti, {Yusuf Valentino}",

note = "Funding Information: This study is supported by the JSPS fellowship to Mostafa Kamal Masud and the Advance Queensland Industry Research Fellowship (AQIRF043-2020RD3) to Yusuf Valentino Kaneti. The authors would like to thank Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudia Arabia for supporting this project. Chia-Hung Liu would like to acknowledge funding support from Ministry of Science and Technology, Taiwan (MOST 110-2222-E-038-002). The MPGE fabrication is performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano-and micro-fabrication facilities for Australian researchers. Publisher Copyright: {\textcopyright} 2022 The Chemical Society of Japan.",

year = "2022",

doi = "10.1246/bcsj.20210286",

language = "English",

volume = "95",

pages = "198--207",

journal = "Bulletin of the Chemical Society of Japan",

issn = "0009-2673",

publisher = "Chemical Society of Japan",

number = "1",

}

TY - JOUR

T1 - κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

AU - Salahuddin, Bidita

AU - Masud, Mostafa Kamal

AU - Aziz, Shazed

AU - Liu, Chia Hung

AU - Amiralian, Nasim

AU - Ashok, Aditya

AU - Hossain, S. M.Azad

AU - Park, Hyeongyu

AU - Wahab, Md Abdul

AU - Amin, Mohammed A.

AU - Chari, M. Adharvana

AU - Rowan, Alan E.

AU - Yamauchi, Yusuke

AU - Hossain, Md Shahriar A.

AU - Kaneti, Yusuf Valentino

N1 - Funding Information: This study is supported by the JSPS fellowship to Mostafa Kamal Masud and the Advance Queensland Industry Research Fellowship (AQIRF043-2020RD3) to Yusuf Valentino Kaneti. The authors would like to thank Taif University Researchers Supporting Project number (TURSP-2020/03), Taif University, Taif, Saudia Arabia for supporting this project. Chia-Hung Liu would like to acknowledge funding support from Ministry of Science and Technology, Taiwan (MOST 110-2222-E-038-002). The MPGE fabrication is performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano-and micro-fabrication facilities for Australian researchers. Publisher Copyright: © 2022 The Chemical Society of Japan.

PY - 2022

Y1 - 2022

N2 - Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs.

AB - Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs.

KW - Gold electrode

KW - Mesoporous metals

KW - MicroRNA sensor

UR - http://www.scopus.com/inward/record.url?scp=85126331773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85126331773&partnerID=8YFLogxK

U2 - 10.1246/bcsj.20210286

DO - 10.1246/bcsj.20210286

M3 - Article

AN - SCOPUS:85126331773

SN - 0009-2673

VL - 95

SP - 198

EP - 207

JO - Bulletin of the Chemical Society of Japan

JF - Bulletin of the Chemical Society of Japan

IS - 1

ER -

κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this