TY - JOUR
T1 - β-Endorphin
T2 - Deletion of a single amino acid residue abolishes immunoreactivity but retains opiate potency
AU - Li, Choh Hao
AU - Chang, Wen Chang
AU - Yamashiro, Donald
AU - Tseng, Liang Fu
N1 - Funding Information:
This work was supported in part by grants to C.H.L.) and NIDA (DA-01314 to L-F.T.).
PY - 1979/4/13
Y1 - 1979/4/13
N2 - Two synthetic analogs of camel β-endorphin, one with omission of Leu-14 and the other with omission of Asn-20, have been assayed for immunoreactivity by radioimmunoassay, opiate activity in the guinea pig ileum preparation and analgesic potency in mice. It was found that the omission analogs had no immunoreactivity, but retained significant biological activities. As far as we are aware, this is the first instance in which deletion of a single amino acid residue in a biologically active peptide abolished immunoreactivity.
AB - Two synthetic analogs of camel β-endorphin, one with omission of Leu-14 and the other with omission of Asn-20, have been assayed for immunoreactivity by radioimmunoassay, opiate activity in the guinea pig ileum preparation and analgesic potency in mice. It was found that the omission analogs had no immunoreactivity, but retained significant biological activities. As far as we are aware, this is the first instance in which deletion of a single amino acid residue in a biologically active peptide abolished immunoreactivity.
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U2 - 10.1016/0006-291X(79)92014-X
DO - 10.1016/0006-291X(79)92014-X
M3 - Article
C2 - 454419
AN - SCOPUS:0018760353
SN - 0006-291X
VL - 87
SP - 693
EP - 697
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -